Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASR-006), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Scientific background
Somatostatin is a small cyclic peptide that is widely expressed throughout the central nervous system and peripheral tissues.1 In peripheral tissues, somatostatin exerts inhibitory effects on secretion processes, whereas in the brain, it acts as a neurotransmitter in both a stimulatory and an inhibitory manner1,2.
Somatostatin mediates its action via six high affinity G-protein coupled receptors (SSTR1, SSTR2a, SSTR2b, SSTR3, SSTR4, and SSTR5), which are encoded by five genes1,2. Expression of the different receptors is developmentally regulated in a time- and tissue-specific manner2.
Somatostatin receptors have been found on a variety of neuroendocrine tumors, such as paragangliomas, carcinoids, and breast tumors3. Synthetic peptide derivatives of somatostatin have been successfully used in the treatment of neuroendocrine malignancies and in vivo imaging of tumors that are positive for somatostatin receptors4.
In general, SSTR2 is the most common SSTR subtype found in human tumors, followed by SSTR1, with SSTR3 and SSTR4 being less common.
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
