Overview
- Peptide (C)KRRTPHAT(S)FNGED, corresponding to amino acid residues 652 - 665 of human SORL1 (Accession Q92673). Extracellular, N-terminus.
- Rat and mouse brain membranes; human Jurkat T-cell leukemia, SH-SY5Y neuroblastoma, Colo-205 colon adenocarcinoma, and HepG2 hepatocellular carcinoma cell lysates (1:200-1:1000).
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1, 2. Anti-SORL1 (extracellular) Antibody (#ANR-132), (1:200).
3, 4. Anti-SORL1 (extracellular) Antibody, preincubated with SORL1 (extracellular) Blocking Peptide (#BLP-NR132).
- Human Jurkat T-cell leukemia cells (5 µg).
Sortilin-related receptor1 (SORL1, also known as LR11 or SORLA) is a receptor involved in intracellular sorting and trafficking of proteins into their respective subcellular compartments1.
Direction of proteins into their proper compartments is essential for normal cell function and defects in these pathways are thought to be important factors in the pathogenesis of Alzheimer’s disease (AD).
The full-length transcript of SORL1 encodes a 250 kDa, membrane-bound protein comprised of multiple functional domains. These include a vacuolar protein sorting 10 (VPS10) domain, five low-density lipoprotein receptor (LDLR) class B repeats, an epidermal growth factor-like (EGF-like) domain, eleven LDLR class A repeats, six fibronectin-type (FN-type) repeats, a transmembrane domain and a cytosolic domain containing recognition motifs for cytosolic adaptors1.
SORL1 is widely expressed in the human brain, particularly in neurons of the hippocampus and some nuclei of the brainstem and Purkinje cells, and has slightly weaker expression in neurons of the thalamus and the hypothalamus2. It is also expressed in other tissue types such as the testes, ovaries, thyroid, and lymph nodes3.
