Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.95 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*
Western blot
Rat heart and kidney (1:200). Human brain neuroblastoma (SH-SY5Y) and MS-1 cell lysate. It is recommended to add 0.5% Tween-20 to blocking and antibody solutions.
Western blot analysis of rat kidney (lanes 1 and 3), rat heart (lanes 2 and 4), human SH-SY5Y brain neuroblastoma (lanes 5 and 7) and Human MS-1 neuroendocrine skin carcinoma cell (lanes 6 and 8) lysates:
Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2.
Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.
Sphingosine 1-phosphate receptor 2 (S1PR2) is ubiquitously expressed. S1PR2 mRNA was detected in the brain, heart, spleen, liver, lung, thymus, kidney and skeletal muscle4. Unlike S1PR1, knockout of SIPR2 gene is not lethal5.
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Western blot analysis of rat kidney (lanes 1 and 3), rat heart (lanes 2 and 4), human SH-SY5Y brain neuroblastoma (lanes 5 and 7) and Human MS-1 neuroendocrine skin carcinoma cell (lanes 6 and 8) lysates:1,2,5,6. Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:200).
