Overview
- Peptide SAEKQWEVPDTASEC, corresponding to amino acid residues 583-597 of human STIM2 (Accession Q9P246). Intracellular, C-terminus.
- RBL (rat basophilic leukemia) cell line and rat brain lysates (1:200).
Western blot analysis of rat brain (lanes 1, 3) and rat RBL basophilic leukemia cell (lanes 2, 4) lysates:1. Anti-STIM2 Antibody (#ACC-064), (1:200).
2. Anti-STIM2 Antibody, preincubated with STIM2 Blocking Peptide (#BLP-CC064).
- Rat lung paraffin sections (1:100)
- Rat fixed basophilic leukemia (RBL) cells.
Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become the focus of intense research.
Recently, several key players of the store-operated complex have been identified: the Orai family consists of three members: Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4
While STIM1 appears to be localized intracellularly at the ER membrane and at a much lower extent on the cell surface of several cell types, STIM2 appears to be localized solely at the ER membrane.5 The function of STIM2 still remains elusive and several models have been suggested.6-8
Expression of STIM2 in mouse cortical neurons and human SH-SY5Y cells.Western blot analysis of mouse cortical neuron lysates and human SH-SY5Y cell lysates using Anti-STIM2 Antibody (#ACC-064). Actin is used as a loading control.Adapted from Gonzalez-Sanchez, P. et al. (2017) Front. Cell. Neurosci. 11, 363. with permission of Frontiers.
