Overview
- Peptide (C)RSGYRALPLGDFDR, corresponding to amino acid residues 4-17 of mouse STOML1 (Accession Q8CI66). Extracellular, N-terminus.
- Western blot analysis of rat dorsal root ganglia lysates:1. Anti-Stomatin-like Protein 1 (extracellular) Antibody (#AIP-001), (1:500).
2. Anti-Stomatin-like Protein 1 (extracellular) Antibody, preincubated with Stomatin-like Protein 1 (extracellular) Blocking Peptide (#BLP-IP001). - Western blot analysis of mouse brain lysates:1. Anti-Stomatin-like Protein 1 (extracellular) Antibody (#AIP-001), (1:800).
2. Anti-Stomatin-like Protein 1 (extracellular) Antibody, preincubated with Stomatin-like Protein 1 (extracellular) Blocking Peptide (#BLP-IP001).
- Mairhofer, M. et al. (2009) J. Biol. Chem. 284, 29218.
- Goshe, P. et al. (2010) PLoS Genet. 9, e1004063.
- Kozlenkov, A. et al. (2014) J. Physiol. 592, 557.
The Stomatin-like protein family contains five members and are characterized by the presence of a structurally conserved core called the stomatin-domain. This stomatin-domain is further related to a domain found in members of the prohibitin, flotillin and HflK/HflC protein families and has accordingly also been called the SPFH domain.
All mammalian stomatin proteins are integral membrane protein with a single, relatively short, hydrophobic membrane insertion domain, followed by the core stomatin-domain. A single conserved proline residue (known to be a potent helix breaker) in the hydrophobic region is required for the formation of the protein’s hairpin structure. The N-terminal domain is extracellular. Stomatin-like protein 1 (STOML1) has a typical stomatin-domain at the N-terminus and an additional sterol carrier protein-2 (SCP-2) domain at the C-terminus. STOML-1 is targeted to the late endosomal compartment where it interacts with stomatin. STOML-1 is predominantly expressed in the brain, in a subpopulation of sensory neurons in the dorsal root ganglia. Much lower levels are detected in other tissues1.
Following anoxia, STOML-1 expression is up-regulated by the oxidative response factor SKN-1/Nrf. SKN-1/Nrf is required for both rapid mitochondrial refusion and rapid behavioral recovery during reoxygenation suggesting that STOML-1 helps facilitate mitochondrial dynamics following anoxia2.
STOML-1 also modulates the proton-sensitive members of the acid-sensing ion channel (ASIC) family. STOML-1 strongly inhibits ASIC1a and accelerates the inactivation time constant of ASIC3, but has no effect on the splice variant ASIC1b3.
Application key:
Species reactivity key:
Anti-Stomatin-like Protein 1 (extracellular) Antibody (#AIP-001) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize STOML1 from human, mouse, and rat samples.