Name: Anti-STOML2/SLP-2 Antibody
Brand: Alomone Labs
SKU: AIP-002

Overview

Cat #: AIP-002

Alternative Name Paraprotein target 7, EPB72-like protein 2, Stomatin-like protein 2

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EGTRESAINVAEGKK, corresponding to amino acid residues 208-222 of rat Stomatin-like protein 2 (Accession Q4FZT0). Intracellular.

Accession (Uniprot) Number Q4FZT0

Gene ID 298203

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human – identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

May also work in: ic*, ih*, ip*

Western blot

Western blot analysis of rat brain lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat skeletal muscle lysate (lanes 3 and 6):
1-3. Anti-STOML2/SLP-2 Antibody (#AIP-002), (1:400).
4-6. Anti-STOML2/SLP-2 Antibody, preincubated with STOML2/SLP-2 Blocking Peptide (#BLP-IP002).
Western blot analysis of human brain neuroblastoma SH-SY5Y (lanes 1 and 5), human prostate carcinoma DU 145 (lanes 2 and 6), human pancreatic carcinoma PANC-1 (lanes 3 and 7) and human acute lymphoblastic leukemia MOLT-4 (lanes 4 and 8):
1-4. Anti-STOML2/SLP-2 Antibody (#AIP-002), (1:800).
5-8. Anti-STOML2/SLP-2 Antibody, preincubated with STOML2/SLP-2 Blocking Peptide (#BLP-IP002).

References

Zhu, W. et al. (2018) Med. Sci. Monit. 24, 7625.
Sun, F. et al. (2015) BMC Cancer 15, 746.
Wang, Y. and Morrow, J.S. (2000) J. Biol. Chem. 275, 8062.

Scientific background

Stomatin-like protein 2 (STOML2, SLP-2) is a key protein on the mitochondrial inner membrane and a member of the stomatin superfamily. STOML2 is widely expressed in human tissues such as the brain, lung, and kidneys and interacts with prohibitin-1 and 2. It plays an important role in numerous physiological processes including organizing sphingolipid and cholesterol-rich lipid rafts, regulating ion channel conductance, and linking other integral membrane proteins to the peripheral cytoskeleton^1,2.

STOML2 shares a consensus stomatin signature sequence that defines the stomatin gene family. However, it lacks a NH₂-terminal hydrophobic domain shared by other members³.

Studies show that STOML2 expression is upregulated in various human cancer tissues including gastric cancer, endometrial adenocarcinoma, and breast cancer. The protein may play an important role in the incidence and development of tumor and it is suggested that SLP-2 is a potential oncogene. Knockdown of STOML2 protein significantly restrains the viability, migration, and invasion of hepatoma cell line^1,2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-STOML2/SLP-2 Antibody (#AIP-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Stomatin-like protein 2 from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

STOML2/SLP-2 Blocking Peptide (#BLP-IP002) is the original antigen used for immunization during Anti-STOML2/SLP-2 Antibody (#AIP-002) generation. The blocking peptide binds and ‘blocks’ Anti-STOML2/SLP-2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

STOML2/SLP-2 Blocking Peptide (#BLP-IP002)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Anti-STOML2/SLP-2 Antibody