Name: Anti-Synapsin III (SYN3) Antibody
Brand: Alomone Labs
SKU: ANR-017

Overview

Cat #: ANR-017

Alternative Name Synapsin-3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)RPPVQGRSTSQQGE, corresponding to amino acid residues 510-523 of rat Synapsin III (Accession O70441). Extracellular, C-terminus.

Accession (Uniprot) Number O70441

Gene ID 29130

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human – identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
1,2. Anti-Synapsin III (SYN3) Antibody (#ANR-017), (1:400).
3,4. Anti-Synapsin III (SYN3) Antibody, preincubated with Synapsin III/SYN3 Blocking Peptide (#BLP-NR017).
Western blot analysis human SH-SY5Y neuroblastoma cell line lysate:
1. Anti-Synapsin III (SYN3) Antibody (#ANR-017), (1:200).
2. Anti-Synapsin III (SYN3) Antibody, preincubated with Synapsin III/SYN3 Blocking Peptide (#BLP-NR017).

Immunohistochemistry

Expression of Synapsin 3 in mouse and rat hippocampus
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Synapsin III (SYN3) Antibody (#ANR-017), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SYN3 staining (green) in mouse hippocampal CA3 region is detected in the mossy fiber terminal (MFT) region. B. In rat hippocampal dentate gyrus (DG) region, SYN3 immunoreactivity (green) is observed in hilus of the dentate gyrus. Cell nuclei are stained with DAPI (blue).

References

Cruceanu, C. et al. (2013) Int. J. Neuropsychopharmacol. 16, 289.
Murphy, B.C. et al. (2008) BMC Med. Genet. 9, 115.
Cheng. Q. et al. (2018) Front. Synaptic Neurosci. 10, 33.
Gerth, F. et al. (2017) Proc. Natl. Acad. Sci. U.S.A. 114, 12057.

Scientific background

Synapsin 3 (Syn3) belongs to the synapsin family of neuronal phosphoproteins, vesicle-associated proteins that includes two additional members: Synapsin I and Synapsin II. Alternative splicing of the genes results in at least ten variants with various levels of homology.

Synapsins are involved in synaptogenesis, synaptic transmission and synaptic plasticity and are required for the formation and maintenance of the reserve and recycling pools of synaptic vesicles through association with the actin cytoskeleton. Several protein kinases, including PKA, calcium-calmodulin regulated protein kinases and the mitogen-activated protein kinase (MAPK) use synapsins as their substrates^1-4.

Syn3 plays an important role in several disorders, including bipolar disorder, schizophrenia and epilepsy¹.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Synapsin III (SYN3) Antibody (#ANR-017) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Synapsin III from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Synapsin III/SYN3 Blocking Peptide (#BLP-NR017) is the original antigen used for immunization during Anti-Synapsin III (SYN3) Antibody (#ANR-017) generation. The blocking peptide binds and ‘blocks’ Anti-Synapsin III/SYN3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Synapsin III/SYN3 Blocking Peptide (#BLP-NR017)

Shipping and Ordering Information

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Anti-Synapsin III (SYN3) Antibody