Name: Anti-Synaptotagmin-1 (SYT1) Antibody
Brand: Alomone Labs
SKU: ANR-003
Rating: 4.7 (14 reviews)

Overview

Cat #: ANR-003

Alternative Name Synaptotagmin I, SytI, p65, SVP65

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: m, r

May also work in: h*

Immunogen

Peptide MVSASHPEALAAPVTTVAT(C), corresponding to amino acid residues 1-19 of rat synaptotagmin 1 (Accession P21107). Intravesicular, N-terminus.

Accession (Uniprot) Number P21107

Gene ID 25716

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 18/19 amino acid residues identical; human, bovine - 17/20 amino acid residues identical; chicken - 15/19 amino acid residues identical.

RRID AB_2040222.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.2 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
1,2. Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400).
3,4. Anti-Synaptotagmin-1 (SYT1) Antibody, preincubated with Synaptotagmin-1/SYT1 Blocking Peptide (#BLP-NR003).

Immunohistochemistry

Expression of Synaptotagmin-I in mouse hippocampus
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003), (1:400), followed by anti-rabbit-Alexa-488 antibody. Synaptotagmin-I staining (green) appears in the hilus of the hippocampal dentate gyrus. Nuclei are stained with DAPI (blue).

References

Südhof, T.C. (2002) J. Biol. Chem. 277, 7629.
Malsam, J. et al. (2008) Cell. Mol. Life Sci. 65, 2814.
Meir, A. et al. (1999) Physiol. Rev. 79, 1019.

Scientific background

Synaptotagmin 1 is a member of the Ca²⁺-binding synaptotagmin family of proteins that includes 16 members in vertebrates and is involved in regulated exocytosis and trafficking^1,2.

Synaptotagmins are type I membrane proteins located in intracellular vesicles and consist of a short N-terminal domain located in the vesicle lumen, a transmembrane domain and a cytosolic domain containing two Ca²⁺ binding domains^1,2.

Synaptotagmins are usually localized to distinct secretory vesicles and control their Ca²⁺-dependent fusion. Synaptotagmin I is predominantly expressed in neuronal cells and is an abundant constituent of synaptic vesicles^1,2.

Synaptotagmin I has a relatively low Ca²⁺ affinity and is able to bind Ca²⁺ mostly in the vicinity of Ca²⁺ channels pores. Indeed, Synaptotagmin I has been demonstrated to directly interact with selected Ca²⁺ channels. Synaptotagmin I also binds to the t-SNARE protein Syntaxin 1 in a Ca²⁺-dependent manner thus forming the prototypical Ca²⁺-dependent fast synaptic vesicle exocytosis protein complex that also includes SNAP-25 and VAMP^1,2,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against the intravesicular N-terminal domain of rat synaptotagmin 1. Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize synaptotagmin 1 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Immunocytochemistry citations

Rat PC12 cells.
Peixoto-Neves, D. et al. (2018) Biochem. Biophys. Res. Commun. 506, 709.

More product citations

Zhao, X.S. et al. (2001) EMBO J. 20, 2680.
Shin, D.M. et al. (2000) J. Cell Biol. 150, 1101.
Barbosa, J. et al. (1999) J. Neurochem. 73, 1881.

Specifications

Scientific Background

Specific Control Product

Synaptotagmin-1/SYT1 Blocking Peptide (#BLP-NR003) is the original antigen used for immunization during Anti-Synaptotagmin-1 (SYT1) Antibody (#ANR-003) generation. The blocking peptide binds and ‘blocks’ Anti-Synaptotagmin-1/SYT1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Synaptotagmin-1/SYT1 Blocking Peptide (#BLP-NR003)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Anti-Synaptotagmin-1 (SYT1) Antibody