Overview
- Peptide KDRLEQLKAKQLTQD(C), corresponding to amino acid residues 2-16 of rat syntaxin 3 (Accession Q08849). Cytoplasmic, N-terminus.
Western blot analysis of rat brain (lanes 1 and 3) and kidney (lanes 2 and 4) membranes:1,2. Anti-Syntaxin 3 Antibody (#ANR-005), (1:200).
3,4. Anti-Syntaxin 3 Antibody, preincubated with Syntaxin 3 Blocking Peptide (#BLP-NR005).
- Human MDA-MB-231 cells (Williams, K.C. et al. (2014) Mol. Biol. Cell 25, 2061.).
- Jahn, R. and Scheller, R.H. (2006) Nat. Rev. Mol. Cell Biol. 7, 631.
- Südhof, T.C. and Rothman, J.E. (2009) Science 323, 474.
- Weisz, O.A. and Rodriguez-Boulan, E. (2009) J. Cell. Sci. 122, 4253.
Syntaxin 3 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60–70 amino acids that are arranged in heptad repeats1,2. SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.
SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.
Syntaxin 3, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.
Syntaxin 3 functions as a plasma membrane protein t-SNARE that is involved in epithelial cell polarization. Indeed, Syntaxin 3 is specifically localized in the apical membrane of polarized epithelial cells where it likely has a role in the polarized trafficking of newly synthesized proteins3.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Syntaxin 3. Anti-Syntaxin 3 Antibody (#ANR-005) can be used in western blot and immunocytochemistry applications. It has been designed to recognize Syntaxin 3 from rat, mouse and human samples.
Applications
Citations
Powered by Bioz- Western blot of human AD293 and Caco-2 cells. Tested in cells transfected with Syntaxin 3 (STX3) siRNA.
Motoike, S. et al. (2021) J. Pharmacol. Sci. 145, 297.
- Human MDA-MB-231 cells.
Williams, K.C. et al. (2014) Mol. Biol. Cell 25, 2061.
