Overview
- Peptide (C)DVAIRHVQSMYEEAYSD, corresponding to amino acid residues 114 - 130 of mouse TSPAN2 (Accession Q922J6). Extracellular, 2nd loop.
Tetraspanin 2 (extracellular) Blocking Peptide (#BLP-NR188)
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-Tetraspanin 2 (extracellular) Antibody (#ANR-188), (1:200).
3-4. Anti-Tetraspanin 2 (extracellular) Antibody, preincubated with Tetraspanin 2 (extracellular) Blocking Peptide (BLP-NR188).
Western blot analysis of mouse BV-2 microglia cell line lysate (lanes 1 and 3) and mouse P815 mastocytoma cell line lysate (lanes 2 and 4):1-2. Anti-Tetraspanin 2 (extracellular) Antibody (#ANR-188), (1:200).
3-4. Anti-Tetraspanin 2 (extracellular) Antibody, preincubated with Tetraspanin 2 (extracellular) Blocking Peptide (BLP-NR188).
Western blot analysis of human K562 chronic myelogenous leukemia cell line lysate (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysate (lanes 2 and 4):1-2. Anti-Tetraspanin 2 (extracellular) Antibody (#ANR-188), (1:200).
3-4. Anti-Tetraspanin 2 (extracellular) Antibody, preincubated with Tetraspanin 2 (extracellular) Blocking Peptide (BLP-NR188).
Expression of Tetraspanin 2 (TSPAN2) in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-Tetraspanin 2 (extracellular) Antibody (#ANR-188), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. TSPAN2 immunoreactivity (green), appears in cells of layers I and II (vertical arrows) and in cells of deeper layers (horizontal arrows). B. Pre-incubation of the antibody with Tetraspanin 2 (extracellular) Blocking Peptide (BLP-NR188), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Tetraspanin 2 (TSPAN2) in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Tetraspanin 2 (extracellular) Antibody (#ANR-188), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. TSPAN2 immunoreactivity (green), appears in cells of the purkinje layer (horizontal arrows) and in cells of the molecular layer (vertical arrows). B. Pre-incubation of the antibody with Tetraspanin 2 (extracellular) Blocking Peptide (BLP-NR188), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer, M = molecular layer
Cell surface detection of Tetraspanin 2 (TSPAN2) by indirect flow cytometry in live intact mouse BV-2 microglia cell line:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-Tetraspanin 2 (extracellular) Antibody (#ANR-188), (2.5μg) + goat-anti-rabbit-FITC.
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Tetraspanin 2 (TSPAN2) is a member of the Tetraspanin family of proteins, that comprises 33 members in humans. The family is characterized by the presence of four transmembrane domains, which give rise to the name "tetraspanin”, as well as the presence two extracellular loops (EC1 and EC2), a small cytoplasmic loop, and short N- and C-terminal tails 1,2.
Tetraspanins are able to interact with each other and also with numerous partner proteins such as integrins, growth factor receptors and ion channels, to create a dynamic network of interactions known as the tetraspanin-enriched microdomains (TEMs). Consequently, tetraspanins are regarded as molecular organizers and by forming complexes with these proteins, tetraspanins can modulate signal transduction pathways and cellular responses 2,3,4.
Although TSPAN2 shares a relatively high homology with well-studied tetraspanins like CD9 (TSPAN29) or CD81 (TSPAN28), it has been less well-characterised up until now 3.
Nevertheless, TSPAN2, like the related CD9 and CD81 proteins, has been described in relation to exosome formation, specifically regarding neuroinflammation and microglia activation 5, as well as oligodendrocyte differentiation into myelin-forming glia and in the progression of tumor metastasis by modulating cancer cell motility and invasion 3,5.
Anti-Tetraspanin 2 (extracellular) Antibody (#ANR-188) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize TSPAN2 from mouse, rat and human samples.
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