Overview
- Peptide (C)RTYTDAMQTYNGNDER, corresponding to amino acid residues 122 - 137 of human Tetraspanin 7 (Accession P41732). Extracellular, 2nd loop.
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:500). 3-4. Anti-Tetraspanin 7 (extracellular) Antibody, preincubated with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 4):1-2. Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:400). 3-4. Anti-Tetraspanin 7 (extracellular) Antibody, preincubated with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187).
Expression of Tetraspanin 7 in the rat substantia nigra region.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Tetraspanin 7 immunoreactivity (green) appears in neuronal profiles (arrows) in both pars reticulata (SNR) and pars compacta (SNC). B. Pre-incubation of the antibody with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Tetraspanin 7 in the mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA1 region shows Tetraspanin 7 immunoreactivity (green) in neuronal profiles in the pyramidal (P) and stratum radiatum (SR, horizontal arrows) layers. Glial profiles appeared mainly in the stratum radiatum layer (vertical arrows). B. Pre-incubation of the antibody with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, SO = stratum oriens, SR = stratum radiatum.
Cell surface detection of Tetraspanin 7 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (2.5µg) + goat-anti-rabbit-FITC.
- Termini, C.M. et al. (2017) Front Cell Dev Biol, 5, 34
- Brieuc, P.P. et al. (2020) Med. Microbiol. Immunol. 209, 427.
- McLaughlin, K.A. et al. (2020) Med. Microbiol. Immunol. 209, 437.
Tetraspanins (Tspans) are a family of four-span transmembrane proteins, known as plasma membrane “master organizers.” They form Tspan-enriched microdomains (TEMs or TERMs) through lateral association with one another and other membrane proteins.1
Tetraspanins are characterized by four transmembrane domains, two extracellular loops: one small extracellular loop (SEL or EC1) and one larger extracellular loop (LEL or EC2), a short intracellular loop, and two short intracellular tails.1
Teatraspanin 7 (TSPAN7) is highly expressed in the brain and was first described as a candidate gene for X‐linked intellectual disability, probably through the modulation of surface expression of AMPA and dopamine receptors.2
Tetraspanin 7 also has a key role in controlling pancreatic β-cell Ca2+ handling and defining the set-point of insulin secretion through modulation of L-type Cav1.2 and Cav1.3 channels. Furthermore, it has been identified as a target for autoantibodies in Type I diabetes. 3
In addition, Tetraspanin 7 is involved in the regulation of HIV virus entry into dendritic cells and several types of cancer progression. 2
Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187) is a highly specific antibody directed against an extracellular epitope of human Tetraspanin 7. The antibody can be used in western blot, indirect flow cytometry and immunohistochemistry. It has been designed to recognize Tetraspanin 7 from mouse, rat and human samples.
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