Overview
- Peptide (C)RTYTDAMQTYNGNDER, corresponding to amino acid residues 122 - 137 of human Tetraspanin 7 (Accession P41732). Extracellular, 2nd loop.
- Western blot analysis (1:200-1:800).
Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:500). 3-4. Anti-Tetraspanin 7 (extracellular) Antibody, preincubated with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human HepG2 hepatocellular carcinoma cell line lysate (lanes 2 and 4):1-2. Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:400). 3-4. Anti-Tetraspanin 7 (extracellular) Antibody, preincubated with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187).
Expression of Tetraspanin 7 in the rat substantia nigra region.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Tetraspanin 7 immunoreactivity (green) appears in neuronal profiles (arrows) in both pars reticulata (SNR) and pars compacta (SNC). B. Pre-incubation of the antibody with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of Tetraspanin 7 in the mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal CA1 region shows Tetraspanin 7 immunoreactivity (green) in neuronal profiles in the pyramidal (P) and stratum radiatum (SR, horizontal arrows) layers. Glial profiles appeared mainly in the stratum radiatum layer (vertical arrows). B. Pre-incubation of the antibody with Tetraspanin 7 (extracellular) Blocking Peptide (BLP-NR187), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, SO = stratum oriens, SR = stratum radiatum.
Cell surface detection of Tetraspanin 7 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-Tetraspanin 7 (extracellular) Antibody (#ANR-187), (2.5µg) + goat-anti-rabbit-FITC.
Tetraspanins (Tspans) are a family of four-span transmembrane proteins, known as plasma membrane “master organizers.” They form Tspan-enriched microdomains (TEMs or TERMs) through lateral association with one another and other membrane proteins.1
Tetraspanins are characterized by four transmembrane domains, two extracellular loops: one small extracellular loop (SEL or EC1) and one larger extracellular loop (LEL or EC2), a short intracellular loop, and two short intracellular tails.1
Teatraspanin 7 (TSPAN7) is highly expressed in the brain and was first described as a candidate gene for X‐linked intellectual disability, probably through the modulation of surface expression of AMPA and dopamine receptors.2
Tetraspanin 7 also has a key role in controlling pancreatic β-cell Ca2+ handling and defining the set-point of insulin secretion through modulation of L-type Cav1.2 and Cav1.3 channels. Furthermore, it has been identified as a target for autoantibodies in Type I diabetes. 3
In addition, Tetraspanin 7 is involved in the regulation of HIV virus entry into dendritic cells and several types of cancer progression. 2
