Name: Anti-Thromboxane A2 Receptor (extracellular) Antibody
Brand: Alomone Labs
SKU: APR-095

Overview

Cat #: APR-095

Alternative Name Prostanoid TP receptor, TXA2-R, TBXA2R

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)RPTNITLEERRLIAS, corresponding to amino acid residues 13 - 27 of human TBXA2R (Accession P21731). Extracellular, N-terminus.

Accession (Uniprot) Number P21731

Gene ID 6915

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, rat – 12 out of 15 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody

Applications: ifc, lci, wb

May also work in: ic*, ih*, ip*

Western blot

Western blot analysis of rat spleen lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6):
1-3. Anti-Thromboxane A2 Receptor (extracellular) Antibody (#APR-095), (1:200).
4-6. Anti-Thromboxane A2 Receptor (extracellular) Antibody, preincubated with Thromboxane A2 Receptor (extracellular) Blocking Peptide (BLP-PR095).
Western blot analysis of human K562 chronic myelogenous leukemia cell line lysate (lanes 1 and 4), human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 3 and 6):
1-3. Anti-Thromboxane A2 Receptor (extracellular) Antibody (#APR-095), (1:200).
4-6. Anti-Thromboxane A2 Receptor (extracellular) Antibody, preincubated with Thromboxane A2 Receptor (extracellular) Blocking Peptide (BLP-PR095).

Indirect flow cytometry

Cell surface detection of Thromboxane A2 Receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti- Thromboxane A2 Receptor (extracellular) Antibody (#APR-095), (2.5μg) + goat-anti-rabbit-FITC.

References

Yan, A. et al. (2017) Mol. Med. Rep. 16, 2491.
Zhao, Z. et al. (2017) Oncotarget 8, 30030.
Kinsella, B.T. (2016) Wilms Tumor, Brisbane (AU): Codon Publications, Chapter 11.

Scientific background

Thromboxane A2 (TxA2) is a prostanoid that is synthesized from arachidonic acid and plays an important role in haemostasis, a process regulating platelet aggregation and vascular tone. Thromboxane A2 binds to the thromboxane A2 receptor (TBXA2R) to induce strong vasoconstriction in renal arteries.

Thromboxane A2 receptor is a seven-transmembrane G-protein coupled receptor localized on the cell membrane and in intracellular compartments. TBXA2R is expressed in the central nervous system in several types of cells, such as microglia, oligodendrocytes and astrocytes. Imbalances in the levels of the receptor is widely implicated in several cardiovascular, pulmonary, renal and prostate pathologies^1-3.

Thromboxane A2 receptor mediates vasoconstriction and ischemia and its activation is implicated in atherosclerosis and inflammation. Activation of the receptor induces endothelial cell apoptosis by inhibiting Akt phosphorylation and also inhibits endothelial cell migration and angiogenesis by decreasing Akt and eNOS phosphorylation^1-3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-Thromboxane A2 Receptor (extracellular) Antibody (#APR-095) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize TBXA2R from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

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Anti-Thromboxane A2 Receptor (extracellular) Antibody