Overview
- Peptide (C)EHDFPSWMAKILR, corresponding to amino acid residues 679 - 691 of mouse TMC1 (Accession Q8R4P5). Extracellular, 3rd loop.
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1-2. Anti-TMC1 (extracellular) Antibody (#ATC-010), (1:400).
3-4. Anti-TMC1 (extracellular) Antibody, preincubated with TMC1 (extracellular) Blocking Peptide (BLP-TC010).
Western blot analysis of mouse colon lysate (lanes 1 and 3) and rat eye lysate (lanes 2 and 4):1-2. Anti-TMC1 (extracellular) Antibody (#ATC-010), (1:200).
3-4. Anti-TMC1 (extracellular) Antibody, preincubated with TMC1 (extracellular) Blocking Peptide (BLP-TC010).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4), human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 5) and human 132N1 astrocytoma cell line lysate (lanes 3 and 6):1-3. Anti-TMC1 (extracellular) Antibody (#ATC-010), (1:200).
4-6. Anti-TMC1 (extracellular) Antibody, preincubated with TMC1 (extracellular) Blocking Peptide (BLP-TC010).
Cell surface detection of TMC1 by indirect flow cytometry in live intact human
THP-1 monocytic leukemia cell line:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-TMC1 (extracellular) Antibody (#ATC-010), (2.5μg) + goat-anti-rabbit-FITC.
- Mahendrasingam.S (2019), Sci Rep, 9, 1274.
- Pan et al. (2018), Neuron, 99, 736.
- Kawashima et al. (2015), Pflugers Arch, 467, 85.
- Holt, J.R. et al. (2014), Hear Res., 311, 17.
Transmembrane channel-like protein 1 (TMC1) is one of eight mammalian TMC genes. Proteins that belong to this family contain six transmembrane domains with both the C and N termini on the cytoplasmic side of the membrane, as well as a large loop between domains 4 and 5. This multi-pass transmembrane topologic structure suggests roles as a receptor, transporter, channel, or pump1.
TMC1 and the closely related TMC2 gene are assembled as dimers2 and are expressed in neurosensory hair cells of the mouse inner ear where they appear to have a role as pore forming units in mechanoelectrical transduction.
Mutations of the transmembrane channel-like 1 (TMC1) gene can cause dominant and recessive forms of deafness in humans and mice3 and hence, identification of the molecular functions of Tmc genes is broadly significant because it may provide a handle on the fundamental mechanisms that mediate human hearing and balance4.
Anti-TMC1 (extracellular) Antibody (#ATC-010) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize TMC1 from rat, mouse and human samples.
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