Overview
- Peptide(C)EKWDDVKFHGDRTSK, corresponding to amino acid residues 151-165 of rat PEX5-related protein (Accession Q925N3).
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-PEX5L (TRIP8b) Antibody (#APR-070), (1:400).
3,4. Anti-PEX5L (TRIP8b) Antibody, preincubated with PEX5L/TRIP8b Blocking Peptide (#BLP-PR070).
Expression of PEX5-related protein in rat brainImmunohistochemical staining of rat parietal cortex using Anti-PEX5L (TRIP8b) Antibody (#APR-070), (1:300). A. PEX5Rp (green) is labeled in dendritic and axonal profiles (arrows). B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
- Fransen, M. et al. (2008) Biochim. Biophys. Acta 1783, 864.
- Maynard, E.L. et al. (2004) Proteins 55, 856.
- Lewis, A.S. et al. (2009) J. Neurosci. 29, 6250.
- Santoro, B. et al. (2004) J. Neurosci. 24, 10750.
- Ghosh, D. and Berg, J.M. (2010) J. Am. Chem. Soc. 132, 3973.
- Fodor, K. et al. (2012) PLoS Biol. 10, e1001309.
- Lewis, A.S. et al. (2009) J. Neurosci. 29, 6250.
PEX5R (also called TRIP8b, tetratricopetide repeat-containing Rab8b-interacting protein) is a cytoplasmic protein expressed as a family of alternatively spliced isoforms. PEX5R /TRIP8b isoforms contain a large constant domain preceded by a variable region. The C-terminal half of TRIP8b comprises a tetratricopeptide repeat (TPR) protein binding domain1. Analysis of the Pex5p-like protein revealed the presence of a tetratricopeptide repeat (TPR) domain in its C-terminal half consisting of seven TPR motifs. Pex5R is almost exclusively expressed in brain2.
Recent studies provide strong evidence that PEX5R interacts with the carboxyl-terminal region of Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and regulates their cell-surface expression level and cyclic nucleotide dependence3,4. HCN channels are key modulators of neuronal activity by providing the depolarizing cation current involved in rhythmogenesis, dendritic integration, and synaptic transmission. In contrast, Gosh et al. have shown that human PEX5R can bind to peroxisomal targeting signal-1 (PTS1) ligands5.
Furthermore it was shown that enzyme alanine–glyoxylate aminotransferase (AGT) is recognized by PEX5R in the cytoplasm, which allows its subsequent translocation into the peroxisome6. TRIP8b may play a role in both normal neuronal function and in aberrant neuronal excitability associated with neurological diseases7.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat PEX5-related protein. Anti-PEX5L (TRIP8b) Antibody (#APR-070) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PEX5Rp from rat, mouse and human samples.
Powered by Bioz
