Overview
Alternative Name Short transient receptor potential channel 6, Transient receptor protein 6, TRP6
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6 (Accession Q61143). Intracellular, N-terminus.
Accession (Uniprot) Number Q61143
Gene ID 22068
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; human - 15/16 amino acid residues identical.
RRID AB_2040243.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, ip, wb
Western blot
Western blot analysis of rat brain membranes:1. Anti-TRPC6 Antibody (#ACC-017), (1:200).
2. Anti-TRPC6 Antibody, preincubated with TRPC6 Blocking Peptide (#BLP-CC017).- Rat distal pulmonary smooth muscle cell lysate (PASMCs), (Zhang, Y. et al. (2013) Am. J. Physiol. 304, C833.).
Immunoprecipitation
- Human platelets (Hassock, S.R. et al. (2002) Blood 100, 2801.).
Immunohistochemistry
Expression of TRPC6 in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-TRPC6 Antibody (#ACC-017). A. TRPC6 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse anti parvalbumin in the same brain section. C. Confocal merge of TRPC6 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.
Immunocytochemistry
- Human U373 MG cells (1:120) (Barajas, M. et al. (2008) J. Neurosci. Res. 86, 3456.).
Indirect flow cytometry
- Human pulmonary artery smooth muscle cells (PASMCs) (Yu, Y. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101, 13861.).
- The blocking peptide is not suitable for this application.
References
- Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362.
- Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
- Clapham, D.E. (2003) Nature 426, 517.
- Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707.
- Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690.
- Winn. M.P. et al. (2005) Science, 308, 1801.
- Reiser, J. et al. (2005) Nat. Genet. 37, 739.
Scientific background
The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4
The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities:
1. TRPC1.
2. TRPC4 and TRPC5.
3. TRPC3, TRPC6, TRPC7.
4. TRPC2.2,5
They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.
TRPC6 can form heterotetramers with TRPC3 and TRPC7. It is primarily expressed in brain, lung and muscle. High levels of expression of the channel were also found in human platelets. Recently it was reported that TRPC6 is also expressed in the kidney where a mutated channel has been implicated in kidney failure disease.6,7
Lyophilized Powder
Anti-TRPC6 Antibody (#ACC-017) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPC6 from mouse, rat, and human samples.
Image & Title: 
Knockout validation of Anti-TRPC6 Antibody in mouse glomerular lysates.Western blot analysis of mouse glomerular lysates using Anti-TRPC6 Antibody (#ACC-017) in wild type and TRPC6-/- mice. TRPC6 is not detected in knockout mice.Adapted from Kistler, A.D. et al. (2013) J. Biol. Chem. 288, 36598. with permission of the American Society for Biochemistry and Molecular Biology.
Knockout validation of Anti-TRPC6 Antibody in mouse glomerular lysates.Western blot analysis of mouse glomerular lysates using Anti-TRPC6 Antibody (#ACC-017) in wild type and TRPC6-/- mice. TRPC6 is not detected in knockout mice.Adapted from Kistler, A.D. et al. (2013) J. Biol. Chem. 288, 36598. with permission of the American Society for Biochemistry and Molecular Biology.
For research purposes only, not for human use
Last Update: 29/10/2024
Applications
Citations
Citations
Powered by BiozPublished figures using this product
Expression of TRPC6 in human kidney from MN patientsImmunohistochemical staining of human biopsy specimens from MN patients, FSGS patients, and normal kidney tissue from tumor nephrectomy samples using Anti-TRPC6 Antibody (#ACC-017). TRPC6 expression was below detection levels in control kidneys and only slightly induced in FSGS glomeruli, whereas MN kidneys mostly showed moderate to strong positivity for TRPC6.
Adapted from Kistler, A.D. et al. (2013) J. Biol. Chem. 288, 36598. with permission of the American Society for Biochemistry and Molecular Biology.
KO validation citations
- Immunocytochemical staining of mouse renal proximal tubular cells. Tested in TRPC6-/- mice.
Hou, X. et al. (2018) Cell Death Dis. 9, 1015. - Western blot analysis of mouse brain lysate. Tested in Trpc6-/- mice.
Feng, S. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 11011. - Western blot analysis of mouse platelets. Tested in Trpc6-/- mice.
Harper, M.T. et al. (2013) Sci. Signal. 6, ra50. - Western blot analysis of mouse glomerular lysate. Tested in TRPC6-/- mice.
Kistler, A.D. et al. (2013) J. Biol. Chem. 288, 36598.
Western blot citations
- Rat arterial segment lysate.
Jadeja, R.N. et al. (2017) Oncotarget 8, 30706. - Mouse primary skeletal myoblast lysate.
Oh, M.R. et al. (2017) Sci. Rep. 7, 17936. - Mouse heart lysate.
Ohba, T. et al. (2017) PLoS ONE 12, e0187950. - Rat pulmonary artery smooth muscle cells (PASMCs).
Urban, N. et al. (2017) Br. J. Pharmacol. 174, 4099. - Human podocytes.
Zhang, L. et al. (2017) Cell. Physiol. Biochem. 43, 1777. - Rat kidney lysate.
Zhang, L. et al. (2018) Ren. Fail. 40, 266. - Human A549 adenocarcinoma epithelial cell lysate (1:1000).
Yang, L.L. et al. (2017) Oncotarget 8, 5123. - Rat PASMCs.
Jiang, Q. et al. (2016) Am. J. Physiol. 311, C136. - Rat PASMCs.
Urban, N. et al. (2016) Mol. Pharmacol. 89, 197. - Rat kidney lysate.
Ilatovskaya, D.V. et al. (2015) Sci. Rep. 5, 17637. - Mouse caveolar fraction lysate (1:200).
Tabeling, C. et al. (2015) Proc. Natl. Acad. Sci. U.S.A. 112, E1614. - Rat heart lysate (1:100).
Jiang, Y. et al. (2014) Cell Tissue Res. 355, 201. - Mouse podocyte cell lysate.
Roshanravan, H. and Dryer, S.E. (2014) Am. J. Physiol. 306, F1088. - HEK 293 transfected cells.
Wilson, C. and Dryer, S.E. (2014) Am. J. Physiol. 306, F1018. - Rat heart lysate.
Chen, M.S. et al. (2013) Cell. Physiol. Biochem. 32, 951. - Rat glomerular mesangial cells (1:2000).
Zhang, N. and Ji, Z. (2013) Ren. Fail. 35, 971. - Rat glomerular mesangial cells (HBZY-1) (1:1000).
Qiu, G. and Ji, Z. (2013) Clin. Exp. Med. 14, 169. - Mouse brain lysate. Also tested in TRPC6-/- mice.
Feng, S. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 11011. - Mouse platelets. Also tested in Trpc6-/- mice.
Harper, M.T. et al. (2013) Sci. Signal. 6, ra50. - Mouse glomerular lysate. Also tested in TRPC6-/- mice.
Kistler, A.D. et al. (2013) J. Biol. Chem. 288, 36598. - Immortalized mouse podocytes.
Zhu, L. et al. (2013) Am. J. Physiol. 304, F849. - Human platelets.
Harper, M.T. et al. (2013) Sci. Signal. 6, ra50. - Rat distal pulmonary smooth muscle cell lysate (PASMCs).
Zhang, Y. et al. (2013) Am. J. Physiol. 304, C833. - HEK 293 cells expressing mouse TRPC6 (1:200).
Shi, J. et al. (2013) J. Physiol. 591, 2851.
Immunoprecipitation citations
- Human platelets.
Hassock, S.R. et al. (2002) Blood 100, 2801.
Immunohistochemistry citations
- Rat kidney sections.
Zhang, L. et al. (2018) Ren. Fail. 40, 266. - Rat arterial sections.
Jadeja, R.N. et al. (2017) Oncotarget 8, 30706. - Human bladder sections.
Kim, J.M. et al. (2013) Oncogenesis 2, e77. - Human kidney sections.
Kistler, A.D. et al. (2013) J. Biol. Chem. 288, 36598. - Rat kidney.
Sonneveld, R. et al. (2013) Am. J. Pathol. 182, 1196.
Immunocytochemistry citations
- Human A549 adenocarcinoma epithelia cells.
Yang, L.L. et al. (2017) Oncotarget 8, 5123. - Rat primary cultured fetal and neonatal ventricular myocytes (1:100).
Jiang, Y. et al. (2014) Cell Tissue Res. 355, 201. - Rat mesenteric artery smooth muscle cells (1:100).
Brueggemann, L.I. et al. (2014) J. Biol. Chem. 289, 2099. - Rat glomerula mesangial cells (1:200).
Zhang, N. and Ji, Z. (2013) Ren. Fail. 35, 971. - Rat glomerular mesangial cells (HBZY-1) (1:1000).
Qiu, G. and Ji, Z. (2013) Clin. Exp. Med. 14, 169. - HEK 293 cells expressing mouse TRPC6 (1:500).
Shi, J. et al. (2013) J. Physiol. 591, 2851. - Human U373 MG cells (1:120).
Barajas, M. et al. (2008) J. Neurosci. Res. 86, 3456.
More product citations
- Alvarez-Miguel, I. et al. (2017) J. Physiol. 595, 1497.
- He, X. et al. (2017) Proc. Natl. Acad. Sci. U.S.A. 114, E4582.
- Hong, W. et al. (2017) Cell. Physiol. Biochem. 43, 986.
- Liu, N. et al. (2017) Neurosci. Lett. 651, 1.
- Anderson, M. et al. (2013) Am. J. Physiol. 305, C276.
- Kunert Keil, C. et al. (2013) J. Renin-Angiotensin-Aldosterone System 14, 41.
- Takahashi, S. et al. (2008) J. Physiol. 506, 4209.
- Yoshida, J. et al. (2005) Eur. J. Pharmacol. 510, 217.
- Yu, Y. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101, 13861.
- Hassock, S.R. et al. (2002) Blood 100, 2801.
- Trevino, C.L. et al. (2001) FEBS Letters 509, 119.
