Overview
- Peptide (C)NGTDPYKPK(S)PESD, corresponding to amino acid residues 999-1012 of human TRPM2 (Accession O94759). 3rd extracellular loop.
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1, 2. Anti-TRPM2 (extracellular) Antibody (#ACC-128), (1:200).
3, 4. Anti-TRPM2 (extracellular) Antibody, preincubated with TRPM2 (extracellular) Blocking Peptide (#BLP-CC128).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):1, 2. Anti-TRPM2 (extracellular) Antibody (#ACC-128), (1:200).
3, 4. Anti-TRPM2 (extracellular) Antibody, preincubated with TRPM2 (extracellular) Blocking Peptide (#BLP-CC128).
Expression of TRPM2 in rat substantia nigraImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-TRPM2 (extracellular) Antibody (#ACC-128), followed by goat anti-rabbit-AlexaFluor-488. TRPM2 immunoreactivity (green) appears in neurons in the substantia nigra pars compacta (SNC), (arrows). SNR=substantia nigra pars reticulata. Cell nuclei are stained with DAPI (blue).
Cell surface detection of TRPM2 in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-TRPM2 (extracellular) Antibody (#ACC-128), (2.5 µg) + goat-anti-rabbit-FITC.
- Fleig, A. et al. (2004) Trends. Pharmacol. Sci. 25, 633.
- Kraft, R. et al. (2005) Pflugers. Arch. 451, 204.
- Huang, C.L. (2004) J. Am. Soc.Nephrol. 15, 1690.
- Montell, C. (2005) Sci. STKE. 2005, re3.
- Du, J. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 7239.
The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3.
The TRPM family consists of eight members: TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2.
The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3.
The C-terminal regions of three TRPM members (TRPM2, TRPM6 and TRPM7) are occupied by enzymatic domains. Almost all the C-terminal region of TRPM2 has an enzymatic domain with sequence similarities to Nudix hydrolases (cleave mononucleotide and dinucleotide polyphosphates2,4.
TRPM2 (LTRpC2, TrpC7) is a Ca2+-permeable, nonselective cation channel that is predominantly expressed in various regions of the brain, where it is preferentially localized in microglial cells, the host macrophages of the central nervous system and is also expressed in other tissues, including spleen, heart, liver, lung, and bone marrow. Several splice variants of TRPM2 have been identified5,3.
Anti-TRPM2 (extracellular) Antibody (#ACC-128) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize TRPM2 from mouse, rat, and human samples.
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