Overview
- Peptide CVKDYDLERGPDEK, corresponding to amino acid residues 802-815 of mouse TRPM6 (Accession Q8CIR4). 1st extracellular loop.
Cell surface detection of TRPM6 by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-TRPM6 (extracellular)-FITC Antibody (#ACC-046-F), (2.5µg).
Cell surface detection of TRPM6 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-TRPM6 (extracellular)-FITC Antibody (#ACC-046-F), (5µg).
Cell surface detection of TRPM6 by direct flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
___ Cells + Anti-TRPM6 (extracellular)-FITC Antibody (#ACC-046-F), (5µg).
- Ramsey, S.I. et al. (2006) Annu. Rev. Physiol. 68, 619.
- Pedersen, S.F. et al. (2005) Cell. Calcium. 38, 233.
- Montell, C. (2005) Sci. STKE 2005, re3.
- Hilgemann, D.W. et al. (2001) Sci. STKE 2001, re19.
- Schmitz, C. et al. (2005) J. Biol. Chem. 280, 37763.
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the six TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3
According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC (canonical), TRPV (vanilloid) and TRPM (melastatin). To date, three extra subfamilies are also considered to belong to the TRP family; the TRPA, TRPML, and the TRPP.1-4
The TRPM subfamily consists of eight members, TRPM1 to TRPM8, which also can be further subdivided into four subgroups based on their sequence homology: (1)TRPM1 and TRPM3 (2) TRPM6 and TRPM7 (3) TRPM4 and TRPM5 (4) TRPM2 and TRPM8.5
Anti-TRPM6 (extracellular) Antibody (#ACC-046) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize TRPM6 from mouse, rat and human samples.
Anti-TRPM6 (extracellular)-FITC Antibody (#ACC-046-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize TRPM6 from mouse, rat and human samples.
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