Overview
- Peptide (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1 (Accession O35433). Intracellular, C-terminus.
TRPV1/VR1 Blocking Peptide (BLP-CC030)
Expression of TRPV1 in rat spinal cord dorsal horn region:Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections with Anti-TRPV1 (VR1)-ATTO Fluor-647N Antibody (#ACC-030-FRN), (1:100). A. TRPV1 immunoreactivity (purple) appears in the superficial layer of the dorsal horn (arrows). B. Pre-incubation of the antibody with TRPV1/VR1 Blocking Peptide (BLP-CC030), suppressed staining. Cell nuclei are stained with DAPI (blue).
Multiplex staining of TRPV1 and Neurokinin 1 Receptor (NK1R) in mouse dorsal raphe nucleus:Immunohistochemical staining of perfusion-fixed frozen mouse dorsal raphe nucleus sections using Anti-TRPV1 (VR1)-ATTO Fluor-647N Antibody (#ACC-030-FRN), (1:100) and Anti-Neurokinin 1 Receptor (NK1R) (extracellular)-ATTO Fluor-488 Antibody (#ATR-001-AG), (1:100). A. TRPV1 staining (purple). B. NK1 receptor staining (green). C. Merge of the two images demonstrates co-localization in the medial DRN (blue arrow) and lateral DRN (orange arrow). Cell nuclei are stained with DAPI (blue).
- Gunthorpe, M.J. et al. (2002) Trends Pharmacol. Sci. 23, 183.
- Cesare, P. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 7658.
- Kim, J. et al. (2003) Nature 424, 81.
- Ahern, G. P. et al. (2003) J. Biol. Chem. 278, 30429.
- Ross, R.A. et al. (2003) Br. J. Pharmacol. 140, 790.
- Tominaga, M. et al. (1998) Neuron 21, 531.
- Vlachova, V. et al. (2003) J. Neurosci. 23, 1340.
- Agopyan, N. et al. (2004) Am. J. Physiol. 286, L563.
- Reilly, C.A. et al. (2003) Toxicol. Sci. 73, 170.
TRPV1 (also named VR1, capsaicin receptor and vanilloid receptor) is a member of the transient receptor potential (TRP) channel family, which includes TRPC, TRPM, TRPA, TRPP, TRPML and the TRPV subfamilies. The TRPV subfamily consists of six members named, TRPV1-6. The TRPV1 channel is a vanilloid gated, nonselective cation channel.
The channel has sequence homology to the TRP family, and shares a similar predicted structure of six transmembrane domain (TM) with a pore loop between TM5 and TM61. TRPV1 is expressed predominantly in nociceptors and in sensory neurons2,3.
TRPV1 has many activators among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation5,6.
Other members of this family, TRPV2, TRPV3 and TRPV4 also show thermal sensitivity7. Conformational changes in the C-terminus are responsible for many functions such as permeation and gating.
There is also evidence that deletion of the C-terminus causes a loss of sensitivity to any stimuli7. Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis8,9.
Anti-TRPV1 (VR1) Antibody (#ACC-030) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPV1 from mouse, rat, and human samples.
Anti-TRPV1 (VR1)-ATTO Fluor-647N Antibody (#ACC-030-FRN) is directly conjugated to the ATTO Fluor-647N fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as multicolor immunohistochemistry and immunocytochemistry. It has been designed to recognize TRPV1 from mouse, rat, and human samples.
ATTO Fluor 647N belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and exceptionally high stability towards atmospheric ozone. Thus ATTO Fluor 647N is highly suitable for single-molecule detection applications and super-resolution microscopy techniques such as Structured Illumination Microscopy (SIM), Stimulated Emission Depletion (STED), etc.
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