Overview
Cat #:
ACC-030-FRN
Alternative Name Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
ImmunogenPeptide (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1 (Accession
O35433). Intracellular, C-terminus.
Accession (Uniprot) Number O35433
Gene ID 83810
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, guinea pig - 14/15 amino acid residues identical; human - 10/15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Label ATTO-647N. Maximum absorption 646 nm; maximum fluorescence 664 nm. The fluorescence is excited most efficiently in the range 625 - 660 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm. It can be used in flow cytometry (FACS) using the red (637 nm) laser.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Preadsorption Control 1-10 µg peptide per 1 µg antibody
TRPV1/VR1 Blocking Peptide (BLP-CC030) Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-030), and immunohistochemistry (labeled antibody). Applications: if, ih
Scientific background TRPV1 (also named VR1, capsaicin receptor and vanilloid receptor) is a member of the transient receptor potential (TRP) channel family, which includes TRPC, TRPM, TRPA, TRPP, TRPML and the TRPV subfamilies. The TRPV subfamily consists of six members named, TRPV1-6. The TRPV1 channel is a vanilloid gated, nonselective cation channel.
The channel has sequence homology to the TRP family, and shares a similar predicted structure of six transmembrane domain (TM) with a pore loop between TM5 and TM61. TRPV1 is expressed predominantly in nociceptors and in sensory neurons2,3.
TRPV1 has many activators among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation5,6.
Other members of this family, TRPV2, TRPV3 and TRPV4 also show thermal sensitivity7. Conformational changes in the C-terminus are responsible for many functions such as permeation and gating.
There is also evidence that deletion of the C-terminus causes a loss of sensitivity to any stimuli7. Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis8,9.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 03/12/2024
