Overview
Alternative Name Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1 (Accession O35433). Intracellular, C-terminus.
Accession (Uniprot) Number O35433
Gene ID 83810
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, guinea pig - 14/15 amino acid residues identical; human - 10/15 amino acid residues identical.
RRID AB_2313819.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Reconstitution 0.2 ml double distilled water (DDW).
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, wb
May also work in: ip*, n*
Western blot
Western blot analysis of human TRPV1 transfected HEK-293 cells:1. Anti-TRPV1 (VR1) Antibody (#ACC-030), (1:200).
2. Anti-TRPV1 (VR1) Antibody, preincubated with TRPV1/VR1 Blocking Peptide (#BLP-CC030).
Western blot analysis of rat DRG lysate:1. Anti-TRPV1 (VR1) Antibody (#ACC-030), (1:200).
2. Anti-TRPV1 (VR1) Antibody, preincubated with TRPV1/VR1 Blocking Peptide (#BLP-CC030).
Immunohistochemistry
Expression of TRPV1 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-TRPV1 (VR1) Antibody (#ACC-030). A. TRPV1 (red) in DRG neurons. B. Staining with mouse anti-Parvalbumin (green) in the same DRG section. C. Confocal merge of TRPV1 and Parvalbumin demonstrates colocalization (arrows).
Immunocytochemistry
Expression of TRPV1 in rat DRG primary cultureImmunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglia (DRG) primary cells with Anti-TRPV1 (VR1) Antibody (#ACC-030), (1:200). A. Staining followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Nuclear staining using the cell-permeable dye Hoechst 33342. C. Merged image of panels A and B.
Indirect flow cytometry
- Human sperm cells (De Toni, L. et al. (2016) PLoS ONE 11, e016722).
Neutralization
- Human sperm cells (De Toni, L. et al. (2016) PLoS ONE 11, e016722).
References
- Gunthorpe, M.J. et al. (2002) Trends Pharmacol. Sci. 23, 183.
- Cesare, P. et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 7658.
- Kim, J. et al. (2003) Nature 424, 81.
- Ahern, G. P. et al. (2003) J. Biol. Chem. 278, 30429.
- Ross, R.A. et al. (2003) Br. J. Pharmacol. 140, 790.
- Tominaga, M. et al. (1998) Neuron 21, 531.
- Vlachova, V. et al. (2003) J. Neurosci. 23, 1340.
- Agopyan, N. et al. (2004) Am. J. Physiol. 286, L563.
- Reilly, C.A. et al. (2003) Toxicol. Sci. 73, 170.
Scientific background
TRPV1 (also named VR1, capsaicin receptor and vanilloid receptor) is a member of the transient receptor potential (TRP) channel family, which includes TRPC, TRPM, TRPA, TRPP, TRPML and the TRPV subfamilies. The TRPV subfamily consists of six members named, TRPV1-6. The TRPV1 channel is a vanilloid gated, nonselective cation channel.
The channel has sequence homology to the TRP family, and shares a similar predicted structure of six transmembrane domain (TM) with a pore loop between TM5 and TM6.1 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.2,3
TRPV1 has many activators among them heat, protons, vanilloids like capsaicin, resiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.5,6
Other members of this family, TRPV2, TRPV3 and TRPV4 also show thermal sensitivity.7 Conformational changes in the C-terminus are responsible for many functions such as permeation and gating.
There is also evidence that deletion of the C-terminus causes a loss of sensitivity to any stimuli.7 Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9
Lyophilized Powder
Anti-TRPV1 (VR1) Antibody (#ACC-030) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPV1 from mouse, rat, and human samples.
Image & Title:
Knockout validation of Anti-TRPV1 (VR1) Antibody in mouse adipose tissue lysate.Western blot analysis of mouse adipose tissue lysate using Anti-TRPV1 (VR1) Antibody (#ACC-030). TRPV1 is not detected in TRPV1-/- animals.Adapted from Chen, J. et al. (2015) Cardiovasc. Diabetol. 14, 22. with permission of BioMed Central.
For research purposes only, not for human use
Last Update: 29/10/2024
Applications
Citations
Citations
Powered by BiozPublished figures using this product
Expression of TRPV1 in mouse DRGsImmunohistochemical staining of mouse DRG sections using Anti-TRPV1 (VR1) Antibody (#ACC-030). TRPV1 positive cells are detected in green.
Adapted from Lin, J.G. et al. (2015) PLoS ONE 10, e0128037. with permission of PLoS.
Differential expression of TRPV1 in C57BL/6 and BALB/c mice TG neurons.Immunohistochemical staining of trigeminal (TG) sections from C57BL/6 and BALB/c mice using Anti-TRPV1 (VR1) Antibody (#ACC-030). TRPV1 staining (green) is higher in IB4-positive TG neurons from C57BL/6 than BALB/c mouse strains.
Adapted from Ono, K. et al. (2015) with permission of American Physiological Society.
TRPV1 co-localizes with CGRP in mouse DRG neurons.Immunocytochemical staining of mouse DRG neurons using Anti-TRPV1 (VR1) Antibody (#ACC-030). TRPV1 (green) co-localizes with CGRP (red) in large dense core vesicles (LDCVs).
Adapted from Devesa, I. et al. (2014) with permission of the National Academy of Sciences, USA.
KO validation citations
- Western blot analysis of mouse adipose tissue lysate. Tested in TRPV1-/- mice.
Chen, J. et al. (2015) Cardiovasc. Diabetol. 14, 22.
Western blot citations
- Mouse myoblasts (1:1000).
Obi, S. et al. (2017) J. Appl. Physiol. 122, 683. - Human sperm lysate.
De Toni, L. et al. (2016) PLoS ONE 11, e016722. - Mouse DRG lysate (1:1000).
Khoutorsky, A. et al. (2016) Proc. Natl. Acad. Sci. U.S.A. 113, 11949. - Human placenta lysate (1:500).
Martinez, N. et al. (2016) Placenta 40, 25. - Mouse adipose tissue lysate. Also tested in TRPV1-/- mice.
Chen, J. et al. (2015) Cardiovasc. Diabetol. 14, 22. - Rat brain lysate.
Nam, J.H. et al. (2015) Brain 138, 3610. - Human brain lysate.
Nam, J.H. et al. (2015) Brain 138, 3610. - Mouse brain stem and spinal cord lysates (1:500).
Kim, H.S. et al. (2014) Carcinogenesis 35, 1652. - Human peripheral blood mononuclear cells (PBMCs) and Natural Killer cells (NK) (1:500).
Kim, H.S. et al. (2014) Carcinogenesis 35, 1652.
Immunohistochemistry citations
- Rat DRG sections (1:500).
Morgan, M. et al. (2019) Bone 123, 168. - Human testis sections.
De Toni, L. et al. (2016) PLoS ONE 11, e016722. - Rat DRG sections (1:100).
Doppler, K. et al. (2016) Brain 139, 2617. - Human placenta sections (1:100).
Martinez, N. et al. (2016) Placenta 40, 25. - Mouse DRG sections.
Lin, J.G. et al. (2015) PLoS ONE 10, e0128037. - Rat brain sections.
Nam, J.H. et al. (2015) Brain 138, 3610. - Human brain sections.
Nam, J.H. et al. (2015) Brain 138, 3610. - Mouse trigeminal sections (1:400).
Ono, K. et al. (2015) J. Neurophysiol. 113, 3345. - Rat trigeminal sections (1:500).
Urata, K. et al. (2015) J. Dent. Res. 94, 446. - Human uveal melanoma sections (1:200).
Mergler, S. et al. (2014) Cell. Signal. 26, 56. - Rat DRG sections (1:1000).
Isensee, J. et al. (2014) J. Cell Sci. 127, 216. - Human skin sections (1:1000).
Cheng, H.T. et al. (2013) J. Pain. 14, 941.
Immunocytochemistry citations
- Mouse myoblasts.
Obi, S. et al. (2017) J. Appl. Physiol. 122, 683. - Human sperm cells.
De Toni, L. et al. (2016) PLoS ONE 11, e016722. - Mouse DRG neurons.
Devesa, I. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. 111, 18345.
Indirect flow cytometry citations
- Human sperm cells.
De Toni, L. et al. (2016) PLoS ONE 11, e016722.
Neutralization citations
- Human sperm cells.
De Toni, L. et al. (2016) PLoS ONE 11, e016722.
More product citations
- Tanaka, Y. et al. (2016) Neuron 90, 1215.
- Belghiti, M. et al. (2013) J. Biol. Chem. 288, 9675.
- Efendiev, R. et al. (2013) J. Biol. Chem. 288, 3929.
- Jankowski, M.P. et al. (2013) J. Neurophysiol. 109, 2374.
- Lotteau, S. et al. (2013) PLoS ONE 8, e58673.
- Matsuura, S. et al. (2013) PLoS ONE 8, e52840.
- Jankowski, M.P. et al. (2012) J. Neurosci. 32, 17869.
