Name: Anti-TRPV4-ATTO Fluor-550 Antibody
Brand: Alomone Labs
SKU: ACC-034-AO

Overview

Cat #: ACC-034-AO

Alternative Name OTRPC4, VRL-2, VR-OAC, TRP12

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8). Intracellular, C-terminus.

Accession (Uniprot) Number Q9ERZ8

Gene ID 66026

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical.

RRID AB_2040262.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-034), and immunohistochemistry (labeled antibody).

Applications: if, ih

May also work in: ic*

Immunohistochemistry

Expression of TRPV4 in rat DRG
Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections with Anti-TRPV4-ATTO Fluor-550 Antibody (#ACC-034-AO), (1:20). TRPV4 is present in DRG neurons (red). Note that staining is stronger in small cells (arrows). Hoechst 33342 is used as the counterstain.

References

Montell, C. et al. (2002) Mol. Cell. 9, 229.
Clapham, D.E. (2003) Nature 426, 517.
Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
Peng, J.B. et al. (2003) J. Physiol. 551, 729.

Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells¹. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)^1-4. The TRPV subfamily consists of six members, TRPV1-6⁵.

TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland⁶.

Alomone Labs offers a highly specific antibody directed against an intracellular epitope of TRPV4. Anti-TRPV4 Antibody (#ACC-034) can be used in western blot, immunocytochemical, and immunohistochemical applications and recognizes TRPV4 from rat samples.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-TRPV4 Antibody (#ACC-034) is a highly specific antibody directed against an intracellular epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TRPV4 from human, rat, and mouse samples.

Anti-TRPV4-ATTO Fluor-550 Antibody (#ACC-034-AO) is directly labeled with an ATTO-550 fluorescent dye. ATT O dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-TRPV4-ATTO Fluor-550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 13/02/2024

Applications

Citations

KO validation citations

Immunohistochemical staining of mouse kidney sections with #ACC-034. Tested in TRPV4^-/- mice.
Berrout, J. et al. (2012) J. Biol. Chem. 287, 8782.

Immunohistochemistry citations

Rat urothelia tissue sections (1:500).
Heppner, T.J. et al. (2017) Front. Syst. Neurosci. 11, 87.

Specifications

Scientific Background

Specific Control Products

TRPV4 Blocking Peptide (#BLP-CC034) is the original antigen used for immunization during Anti-TRPV4 Antibody (#ACC-034) generation. The blocking peptide binds and ‘blocks’ Anti-TRPV4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

TRPV4 Blocking Peptide (#BLP-CC034)
Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO), was specifically developed to be used as a negative control in immunohistochemistry and surface staining flow cytometry applications, along our line of rabbit primary antibodies conjugated to the ATTO Fluor-550 fluorophore.

Rabbit IgG Isotype Control-ATTO Fluor-550 (#RIC-001-AO)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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Anti-TRPV4-ATTO Fluor-550 Antibody