Name: Anti-TRPV4 (extracellular) Antibody- PE Antibody
Brand: Alomone Labs
SKU: ACC-124-PE

Overview

Cat #: ACC-124-PE

Alternative Name OTRPC4, VRL-2, VR-OAC, TRP12, Osm-9-Like TRP Channel 4

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). Extracellular, 3^rd loop.

Accession (Uniprot) Number Q9ERZ8

Gene ID 66026

Homology Rat – 14 out of 16 amino acid residues identical, mouse – 13 out of 16 amino acid residues identical, human – 11 out of 16 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label R-Phycoerythrin (R-PE)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-124), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of TRPV4 by direct flow cytometry in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-PE (#RIC-001-PE).
^___ Cells + Anti-TRPV4 (extracellular)-PE Antibody (#ACC-124-PE), (5µg).
Cell surface detection of TRPV4 by direct flow cytometry in live intact human U-87 MG glioblastoma cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-PE (#RIC-001-PE).
^___ Cells + Anti-TRPV4 (extracellular)-PE Antibody (#ACC-124-PE), (5µg).
Cell surface multicolor flow cytometry analysis of live intact mouse J774 macrophage cells with TRPV4 and Piezo1:
J774 cells were labeled with Anti-TRPV4 (extracellular)-PE Antibody (#ACC-124-PE), (5µg) and with Anti-Piezo1 (extracellular)-APC Antibody (#APC-087-APC), (5µg).

References

Montell, C. et al. (2002) Mol. Cell. 9, 229.
Clapham, D.E. (2003) Nature 426, 517.
Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
Peng, J.B. et al. (2003) J. Physiol. 551, 729.

Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells¹. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)^1-4. The TRPV subfamily consists of six members, TRPV1-6⁵.

TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland⁶.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-TRPV4 (extracellular) Antibody (#ACC-124) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize TRPV4 from human, rat, and mouse samples.

Anti-TRPV4 (extracellular)-PE Antibody (#ACC-124-PE) is directly conjugated to R-Phycoerythrin (R-PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 19/05/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-PE (#RIC-001-PE), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to Phycoerythrin (PE) fluorophore.

Rabbit IgG Isotype Control-PE (#RIC-001-PE)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-TRPV4 (extracellular) Antibody- PE Antibody