Overview
Alternative Name OTRPC4, VRL-2, VR-OAC, TRP12, Osm-9-Like TRP Channel 4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). Extracellular, 3rd loop.
Accession (Uniprot) Number Q9ERZ8
Gene ID 66026
Homology Rat – 14 out of 16 amino acid residues identical, mouse – 13 out of 16 amino acid residues identical, human – 11 out of 16 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label R-Phycoerythrin (R-PE)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-124), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
Cell surface detection of TRPV4 by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-PE (#RIC-001-PE).
___ Cells + Anti-TRPV4 (extracellular)-PE Antibody (#ACC-124-PE), (5µg).
Cell surface detection of TRPV4 by direct flow cytometry in live intact human U-87 MG glioblastoma cells:___ Cells.
___ Cells + Rabbit IgG Isotype Control-PE (#RIC-001-PE).
___ Cells + Anti-TRPV4 (extracellular)-PE Antibody (#ACC-124-PE), (5µg).
Cell surface multicolor flow cytometry analysis of live intact mouse J774 macrophage cells with TRPV4 and Piezo1:J774 cells were labeled with Anti-TRPV4 (extracellular)-PE Antibody (#ACC-124-PE), (5µg) and with Anti-Piezo1 (extracellular)-APC Antibody (#APC-087-APC), (5µg).
Scientific background
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65.
TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 19/05/2024
Specifications
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