Name: Anti-TRPV4 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: ACC-124-F

Overview

Cat #: ACC-124-F

Alternative Name OTRPC4, VRL-2, VR-OAC, TRP12, Osm-9-Like TRP Channel 4

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). 3^rd extracellular loop.

Accession (Uniprot) Number Q9ERZ8

Gene ID 66026

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – 14/16 amino acid residues identical; mouse – 13/16 amino acid residues identical; human – 12/16 amino acid residues identical.

RRID AB_2876827

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-124), and direct flow cytometry (labeled antibody).

Applications: fc, lci

May also work in: ic*

Direct flow cytometry

Cell surface detection of TRPV4 in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-TRPV4 (extracellular)-FITC Antibody (#ACC-124-F), (2.5 µg).
Cell surface detection of TRPV4 in live intact human THP-1 monocytic leukemia cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control-FITC.
^___ Cells + Anti-TRPV4 (extracellular)-FITC Antibody (#ACC-124-F), (2.5 µg).

References

Montell, C. et al. (2002) Mol. Cell. 9, 229.
Clapham, D.E. (2003) Nature 426, 517.
Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
Peng, J.B. et al. (2003) J. Physiol. 551, 729.

Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells¹. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)^1-4. The TRPV subfamily consists of six members, TRPV1-6⁵.

TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland⁶.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-TRPV4 (extracellular) Antibody (#ACC-124) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize TRPV4 from human, rat, and mouse samples.

Anti-TRPV4 (extracellular)-FITC Antibody (#ACC-124-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

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Anti-TRPV4 (extracellular)-FITC Antibody