Overview
- Peptide (C)EDQSN(S)TVPSYPA(S)RD, corresponding to amino acid residues 647-662 of rat TRPV4 (Accession Q9ERZ8). 3rd extracellular loop.
Western blot analysis of rat brain lysate:1. Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:200).
2. Anti-TRPV4 (extracellular) Antibody, preincubated with TRPV4 (extracellular) Blocking Peptide (#BLP-CC124).
Expression of TRPV4 in rat DRGImmunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 (extracellular) Antibody (# ACC-124). A. TRPV4 labeling appears in neuronal cell bodies. B. Nuclear staining using DAPI as counter stain. C. merged images of A and B.
Cell surface detection of TRPV4 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells +Anti-TRPV4 (extracellular) Antibody (#ACC-124), (2.5μg) + goat-anti-rabbit-FITC.
Expression of TRPV4 in human U-87 cellsCell surface detection of TRPV4 in live intact human brain glioblastoma U-87 cells. Extracellular staining of cells using Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:25), (red) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. DAPI was used as the counterstaining (blue).
Expression of TRPV4 in human U-87 cellsCell surface detection of TRPV4 in live intact human brain glioblastoma U-87 cells. A. Extracellular staining of cells with Anti-TRPV4 (extracellular) Antibody (#ACC-124), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. Merged images of live cells with A.
- Montell, C. et al. (2002) Mol. Cell. 9, 229.
- Clapham, D.E. (2003) Nature 426, 517.
- Moran, M.M. et al. (2004) Curr. Opin. Neurobiol. 14, 362.
- Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.
- Gunthorpe, M.J. et al. (2002) Trends. Pharmacol. Sci. 23, 183.
- Peng, J.B. et al. (2003) J. Physiol. 551, 729.
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells1. According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP)1-4. The TRPV subfamily consists of six members, TRPV1-65.
TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland6.
Anti-TRPV4 (extracellular) Antibody (#ACC-124) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize TRPV4 from human, rat, and mouse samples.
Applications
Citations
Powered by Bioz- Rat brain sections.
Onishi, M. et al. (2018) Physiol. Genomics 50, 272. - Mouse brain sections.
Shavit-Stein, E. et al. (2017) Front. Mol. Neurosci. 10, 42.
- Human microvascular endothelial cells.
Caires, R. et al. (2017) Cell. Rep. 21, 246.
