Name: Anti-TRPV4 Antibody
Brand: Alomone Labs
SKU: ACC-034

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Overview

Cat #: ACC-034

Alternative Name OTRPC4, VRL-2, VR-OAC, TRP12

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8). Intracellular, C-terminus.

Accession (Uniprot) Number Q9ERZ8

Gene ID 66026

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical.

RRID AB_2040264.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ifc, ih, ip, wb

Western blot

Rat brain lysates and ND7/23 Mouse neuroblastoma /rat dorsal root ganglion neurone hybrid cell line lysate (1:200).
Western blot analysis of rat brain lysates:
1. Anti-TRPV4 Antibody (#ACC-034), (1:200).
2. Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).
Western blot analysis of ND7/23 Mouse neuroblastoma /rat dorsal root ganglion neuron hybrid cell line lysate:
1. Anti-TRPV4 Antibody (#ACC-034), (1:200).
2. Anti-TRPV4 Antibody, preincubated with TRPV4 Blocking Peptide (#BLP-CC034).

Immunoprecipitation

Rat and mouse brain lysates (10 μg Ab/0.500 mg whole-protein) (Benfenati, V. et al. (2011) Proc. Natl. Acad. Sci. U.S.A. 108, 2563).

Immunohistochemistry

Rat brain and mouse cerebellum frozen sections.

Immunocytochemistry

Rat brain and mouse cerebellum frozen sections.

Indirect flow cytometry

Human corneal epithelial cells (HCEC) (1:200) (Pan, Z. et al. (2008) Cell Calcium 44, 374.).
The control antigen is not suitable for this application.

Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.¹ According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).^1-4 The TRPV subfamily consists of six members, TRPV1-6.⁵

TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland.⁶

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title:

Knockout validation of Anti-TRPV4 Antibody in mouse kidney.Immunohistochemical staining of mouse kidney sections using Anti-TRPV4 Antibody (#ACC-034) and Anti-Aquaporin 2-ATTO Fluor-550 Antibody (#AQP-002-AO). TRPV4 staining (green, upper panels) is detected in both the connecting tubules (CNT) and the collecting ducts (CCD). AQP2 immunostaining (red) is also observed in the CCD and CNT. TRPV4 staining is not observed in the sections from TRPV4^-/- mice. Adapted from Berrout, J. et al. (2012) J. Biol. Chem. 287, 8782. with permission of American Society for Biochemistry and Molecular Biology.

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Anti-TRPV4 Antibody

Overview

Application key:

Species reactivity key:

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