Name: Anti-TRPV6 Antibody
Brand: Alomone Labs
SKU: ACC-036

Overview

Cat #: ACC-036

Alternative Name Epithelial calcium channel 2, ECaC2, Calcium transport protein 1, CaT1, CaT-like, CaT-L, Transient receptor potential cation channel subfamily V member 6

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)NRGLEDGESWEYQI, corresponding to amino acid residues 712-725 of human TRPV6 (Accession Q9H1D0). Intracellular, C-terminus.

Accession (Uniprot) Number Q9H1D0

Gene ID 55503

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse - 13/14 amino acid residues identical.

RRID AB_2039791.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.75 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, ip, wb

May also work in: ifc*

Western blot

Human prostate cancer cell line, PC3 (1:200). It is recommended to add 0.5% Tween-20 to the antibody solution.
Western blot analysis of Human PC-3 prostate cancer cell lysate:
1. Anti-TRPV6 Antibody (#ACC-036), (1:200).
2. Anti-TRPV6 Antibody, preincubated with TRPV6 Blocking Peptide (#BLP-CC036).

Immunoprecipitation

Human LNCaP cells (Lallet-Daner, H. et al. (2009) Oncogene 28, 1792.).

Immunohistochemistry

Rat and mouse spinal cord frozen sections.

Immunocytochemistry

PC-3 cells (1:100).

Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.¹ According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).^1-4 The TRPV subfamily consists of six members, TRPV1-6.⁵

TRPV6 (CaT1) and TRPV5 (ECaC) are unique members of the TRP family. Both are epithelial calcium channels, form constitutively open channels, share high degree of homology (about 66%) with differences in the N and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV6 and TRPV5 are highly Ca²⁺ selective and are expressed in Ca²⁺ transporting epithelia where they are assumed to have an important role in Ca²⁺ reabsorption. TRPV6 is highly expressed in the placenta, small intestine and kidney.^6,7

While not being expressed in benign prostate tissue, TRPV6 was found to be upregulated in prostate cancer tissue and correlation between expression and tumor grade was shown. Finally, TRPV6 was suggested as prognostic marker and as a promising candidate for therapeutic strategies.^8,9

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title:

Expression of TRPV6 in prostate cancer.Immunohistochemical staining of human prostate tissue using Anti-TRPV6 Antibody (#ACC-036). Very low expression of TRPV6 is observed in normal (right panel) and benign hyperplasia (middle panel) as opposed to significant expression in prostate adenocarcinoma.Adapted from Raphael, M. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. 111, E3870.

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

TRPV6 Blocking Peptide (#BLP-CC036) is the original antigen used for immunization during Anti-TRPV6 Antibody (#ACC-036) generation. The blocking peptide binds and ‘blocks’ Anti-TRPV6 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

TRPV6 Blocking Peptide (#BLP-CC036)

Shipping and Ordering Information

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Certificate of Analysis

Anti-TRPV6 Antibody