Name: Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody
Brand: Alomone Labs
SKU: ATR-006-AG

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Overview

Cat #: ATR-006-AG

Alternative Name Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide ETLQAFDSHYDYTVC, corresponding to amino acid residues 376-390 of rat TSHR (Accession P21463). Extracellular, N-terminus.

Accession (Uniprot) Number P21463

Gene ID 25360

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human - identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ATR-006), and immunohistochemical staining (labeled antibody).

Applications: if, ih

May also work in: ic*, lci*

Immunohistochemistry

Mouse brain sections (1:60).
Expression of TSH receptor in mouse brain
Immunohistochemical staining of TSHR in mouse brain sections using Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody (#ATR-006-AG), (1:60). A. In the cerebellum, TSHR staining (green) appears in the molecular layer (MOL) and purkinje (P) layer. B. In the hippocampus, staining appears in CA3 pyramidal layer (P), (arrows). Nuclei are stained using DAPI (blue).

Scientific background

Thyroid stimulating hormone (TSH), a glycoprotein hormone composed of α and β chains, is produced by thyrotrope cells of the anterior pituitary¹. The TSH receptor (TSHR) plays a prominent role in thyroid physiology and disease. The established biological function of TSHR in the thyroid gland is to regulate synthesis and secretion of thyroid hormones from follicular thyroid cells; it also plays an important role in controlling the growth and development of the thyroid gland².

TSHR is a G-protein coupled receptor and shares the classic structure of the serpentine receptor family (seven membrane spanning segments, an extracellular N-terminus and an intracellular carboxy terminal)³.

TSHR is primarily expressed in the epithelial cells of the thyroid follicles, but TSHR mRNA and protein have been detected in a variety of cell types including brain, testes, kidney, heart, bone, thymus, lymphocytes, adipose tissue and fibroblasts⁴.

Chronic stimulation of the TSHR leads to over activation of the cAMP pathway that in turn causes thyroid hyperplasia and hyperthyroidism. This process occurs in Graves' disease (GD) in which thyroid stimulating antibodies (TSAB) bind the receptor and mimic the action of TSH⁵.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 02/01/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Products

TSH Receptor/TSHR (extracellular) Blocking Peptide (#BLP-TR006) is the original antigen used for immunization during Anti-TSH Receptor (TSHR) (extracellular) Antibody (#ATR-006) generation. The blocking peptide binds and ‘blocks’ Anti-TSH Receptor/TSHR (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

TSH Receptor/TSHR (extracellular) Blocking Peptide (#BLP-TR006)
Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.
Our Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG), was specifically developed to be used as a negative control in immunohistochemistry and surface staining flow cytometry applications, along our line of rabbit primary antibodies conjugated to the ATTO Fluor-488 fluorophore.

Rabbit IgG Isotype Control-ATTO Fluor-488 (#RIC-001-AG)

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

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Certificate of Analysis

Anti-TSH Receptor (TSHR) (extracellular)-ATTO Fluor-488 Antibody