Name: Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody
Brand: Alomone Labs
SKU: AGT-005-AR

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Overview

Cat #: AGT-005-AR

Alternative Name Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, SLC32A1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (Accession O35458). Cytoplasmic, N-terminus.

Accession (Uniprot) Number O35458

Gene ID 83612

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human - identical.

RRID AB_2340980.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AGT-005), and immunohistochemistry (labeled antibody).

Applications: if, ih

May also work in: ic*

Immunohistochemistry

Rat cerebellum (1:100).
Expression of Vesicular GABA Transporter in rat cerebellum
Immunohistochemical staining of rat frozen cerebellum sections using Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody (#AGT-005-AR), (1:100). A. VGAT staining is shown in red. B. DAPI is used as a general cellular marker (blue). C. Merge of A and B reveal that VGAT is most intensely expressed in the granule and pyramidal layer (arrow).

Scientific background

The superfamily of amino acid transporters includes SLC32, SLC36 and SLC38 families. SLC32 has only one member, the vesicular inhibitory amino acid transporter (VIAAT) or vesicular GABA transporter (VGAT)¹.

VGAT is responsible for the uptake of GABA and glycine in exchange of protons, and their release from nerve terminals¹.

The transporter is organized into ten membrane spanning domains, a cytosolic N-terminus and a luminal C-terminal tail². In many regions in the brain, where it is expressed, it is found constitutively phosphorylated, although the biological significance is currently unknown³.

VGAT is expressed on synaptic vesicles of GABAergic and glycinergic neurons^4-6. The transporter is also detected in the pituitary gland and in the pancreas^7,8.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Image & Title:

Multiplex staining of Vesicular GABA Transporter and Gephyrin in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections using Anti-Gephyrin Antibody (#AIP-005), (1:300) and Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody (#AGT-005-AR), (1:80). A. Gephyrin staining (green) appears in the superficial layer of the spinal dorsal horn and some cellular profiles were stained. B. VGAT staining (red) appears in superficial layer and in cell profiles. C. Merge images of A and B reveals co-localization (arrows) in superficial layers and separate expression in some cells. Cell nuclei were stained with DAPI (blue) as the counterstain.

For research purposes only, not for human use

Last Update: 13/02/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005) is the original antigen used for immunization during Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005) generation. The blocking peptide binds and ‘blocks’ Anti-Vesicular GABA Transporter/VGAT primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005)

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Certificate of Analysis

Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody