Name: Anti-Vesicular GABA Transporter (VGAT) Antibody
Brand: Alomone Labs
SKU: AGT-005

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Overview

Cat #: AGT-005

Alternative Name Vesicular inhibitory amino acid transporter, VIAAT, GABA and glycine transporter, SLC32A1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)GEFGGHDKPKITAWE, corresponding to amino acid residues 106-120 of rat vesicular GABA transporter (Accession O35458). Cytoplasmic, N-terminus.

Accession (Uniprot) Number O35458

Gene ID 83612

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human - identical.

RRID AB_2340979.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, wb

May also work in: ifc*, ip*

Western blot

Rat and mouse brain membranes (1:200). Human U-87 MG glyoblastoma lysate (1:200).
Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human U-87 MG glyoblastoma lysates (lanes 3 and 6):
1-3. Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005), (1:200).
4-6. Anti-Vesicular GABA Transporter (VGAT) Antibody, preincubated with Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005).

Immunohistochemistry

Rat cerebellum (1:100).

Immunocytochemistry

Fixed and permeabilized human U-87 cells (1:200).

Scientific background

The superfamily of amino acid transporters includes SLC32, SLC36 and SLC38 families. SLC32 has only one member, the vesicular inhibitory amino acid transporter (VIAAT) or vesicular GABA transporter (VGAT)¹.

VGAT is responsible for the uptake of GABA and glycine in exchange of protons, and their release from nerve terminals¹.

The transporter is organized into ten membrane spanning domains, a cytosolic N-terminus and a luminal C-terminal tail². In many regions in the brain, where it is expressed, it is found constitutively phosphorylated, although the biological significance is currently unknown³.

VGAT is expressed on synaptic vesicles of GABAergic and glycinergic neurons^4-6. The transporter is also detected in the pituitary gland and in the pancreas^7,8.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005) is the original antigen used for immunization during Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGT-005) generation. The blocking peptide binds and ‘blocks’ Anti-Vesicular GABA Transporter/VGAT primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Vesicular GABA Transporter/VGAT Blocking Peptide (#BLP-GT005)

Shipping and Ordering Information

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Certificate of Analysis

Anti-Vesicular GABA Transporter (VGAT) Antibody