Name: Anti-Human ZACN (extracellular) Antibody
Brand: Alomone Labs
SKU: AZC-001

Overview

Cat #: AZC-001

Alternative Name Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h

Immunogen

Peptide CNFELLHFPRDHSN, corresponding to amino acid residues 157-170 of human ZACN (Accession Q401N2). Extracellular, N-terminus.

Accession (Uniprot) Number Q401N2

Gene ID 353174

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Human.

RRID AB_11219142.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ifc, lci, wb

May also work in: ih*, ip*

Western blot

Human pancreatic carcinoma (PANC-1) and human acute T cell leukemia (Jurkat) cell line lysates (1:200).
Western blot analysis of PANC-1 (lanes 1 and 3) and Jurkat (lanes 2 and 4) cell lysates:
1,2. Anti-Human ZACN (extracellular) Antibody (#AZC-001), (1:200).
3,4. Anti-Human ZACN (extracellular) Antibody, preincubated with Human ZACN (extracellular) Blocking Peptide (#BLP-ZC001).

Indirect flow cytometry

Human acute T cell leukemia (Jurkat) intact living cells (5-10µg antibody/ 0.5-1x10⁶ cells).
The blocking peptide is not suitable for this application.

Live cell imaging / Immunocytochemistry

Human living pancreatic adenocarcinoma (PANC-1) cells (1:25).

Scientific background

Over forty homologous proteins belong to the superfamily of ligand-gated ion channels. These members are divided into subfamilies, for example: acetylcholine, serotonin and glycine receptors.

A new member has been added to this superfamily – ZAC (Zinc-activated channel) which is expressed in humans and dogs but not in rodents¹. Like all other ligand-gated ion channels, ZAC has four transmembrane domains and extracellular N- and C-termini. As its name states, the channel is activated by high concentrations of zinc.

ZAC mRNA was found to be expressed in the placenta, spinal cord, stomach, and fetal brain¹. In another study, ZAC was identified using a computer-assisted program and was detected in similar areas (including pancreas and kidney), but also displayed minor discrepancies².

Being that the channel is expressed in human and dog, deciphering its cellular/physiological functions will be challenging.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

For research purposes only, not for human use

Last Update: 29/10/2024

Specifications

Citations

Applications

Scientific Background

Specific Control Product

Human ZACN (extracellular) Blocking Peptide (#BLP-ZC001) is the original antigen used for immunization during Anti-Human ZACN (extracellular) Antibody (#AZC-001) generation. The blocking peptide binds and ‘blocks’ Anti-Human ZACN (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Human ZACN (extracellular) Blocking Peptide (#BLP-ZC001)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

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Anti-Human ZACN (extracellular) Antibody