Name: Anti-ZIP3 (SLC39A3) Antibody
Brand: Alomone Labs
SKU: AZT-003

Overview

Cat #: AZT-003

Alternative Name Zinc transporter ZIP3, Zrt- and Irt-like protein 3

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EADFEKAHRSKK, corresponding to amino acid residues 31-42 of rat ZIP3 (Accession Q5U1X7). 1^st intracellular loop.

Accession (Uniprot) Number Q5U1X7

Gene ID 314637

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human – 11/12 amino acid residues identical.

RRID AB_2756809.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, wb

May also work in: ic*, ifc*, ip*

Western blot

Western blot analysis of mouse lung (lanes 1 and 4), rat brain (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:
1-3. Anti-ZIP3 (SLC39A3) Antibody (#AZT-003), (1:400).
4-6. Anti-ZIP3 (SLC39A3) Antibody, preincubated with ZIP3/SLC39A3 Blocking Peptide (#BLP-ZT003).
Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate (lanes 1 and 3) and human LNCaP prostate adenocarcinoma cell lysate (lanes 2 and 4):
1,2. Anti-ZIP3 (SLC39A3) Antibody (#AZT-003), (1:200).
3,4. Anti-ZIP3 (SLC39A3) Antibody, preincubated with ZIP3/SLC39A3 Blocking Peptide (#BLP-ZT003).

Immunohistochemistry

Expression of Zinc transporter ZIP3 in rat hippocampus
Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-ZIP3 (SLC39A3) Antibody (#AZT-003), (1:400), followed by goat-anti-rabbit-AlexaFluor-488 antibody. ZIP3 staining (green) appears in CA1 neurons in the pyramidal layer (arrow). Nuclei are labeled with DAPI (blue).

References

Jeong, J. et al. (2013) Mol. Aspects. Med. 34, 612.
Kelleher, S.L. et al. (2005) Am. J. Physiol. Cell. Physiol. 288, C1042.

Scientific background

Zinc uptake transporter, ZIP3, belongs to the SLC39 transporter family. ZIP transporters are responsible for zinc transport into the cytoplasm across cellular membranes, either influx from the extracellular space or efflux from intracellular organelles¹. ZIP3 structure includes eight transmembrane (TM) domains with extracellular N- and C-termini¹.

Mammalian ZIP3 is encoded by the SLC39A3 gene and its expression is detected in tissues with unique requirements for Zn²⁺. High levels of ZIP3 are noticed in specialized hormonally-responsive secretory tissues, such as the pancreas, prostate and mammary gland².

Regulation and maintenance of proper concentration and distribution of cellular zinc nutrient by ZIP transporters are essential to the function, metabolism, growth, proliferation and survival of cells. Zinc deficiency can cause growth retardation, cognitive impairment, metabolic disorders, infertility, dysfunction of the immune system, including abnormalities in T-cells, natural killer cells and monocytes, and reduction in antibody formation¹. However, excess zinc can be toxic, therefore, zinc transport plays a crucial role in regulating cellular zinc homeostasis and its levels are tightly regulated by the activity of zinc transporters.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Zinc transporter ZIP3. Anti-ZIP3 (SLC39A3) Antibody (#AZT-003) can be used in western blot and immunohistochemistry applications. It has been designed to recognize ZIP3 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

ZIP3/SLC39A3 Blocking Peptide (#BLP-ZT003) is the original antigen used for immunization during Anti-ZIP3 (SLC39A3) Antibody (#AZT-003) generation. The blocking peptide binds and ‘blocks’ Anti-ZIP3/SLC39A3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

ZIP3/SLC39A3 Blocking Peptide (#BLP-ZT003)

Shipping and Ordering Information

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Anti-ZIP3 (SLC39A3) Antibody