Name: Anti-ZIP10/SLC39A10 (extracellular) Antibody
Brand: Alomone Labs
SKU: AZT-010

Overview

Cat #: AZT-010

Alternative Name Zinc transporter ZIP10, Zrt- and Irt-like protein 10, Solute Carrier Family 39 Member 10

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)EQYEHNRVHKLDRVH, corresponding to amino acid residues 259 - 273 of mouse SLC39A10 (Accession Q6P5F6). Extracellular, N-terminus.

Accession (Uniprot) Number Q6P5F6

Gene ID 227059

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat – identical. Human – 14 out of 15 amino acid identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preadsorption Control 1 µg peptide per 1 µg antibody
ZIP10/SLC39A10 (extracellular) Blocking Peptide (#BLP-ZT010)

Standard quality control of each lot Western blot analysis.

Applications: ifc, ih, lci, wb

May also work in: ic*, ip*

Western blot

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):
1-2. Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010), (1:200).
3-4. Anti-ZIP10/SLC39A10 (extracellular) Antibody, preincubated with ZIP10/SLC39A10 (extracellular) Blocking Peptide (BLP-ZT010).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 4), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 5) and human K562 chronic myelogenous leukemia cell line lysate (lanes 3 and 6):
1-3. Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010), (1:200).
4-6. Anti-ZIP10/SLC39A10 (extracellular) Antibody, preincubated with ZIP10/SLC39A10 (extracellular) Blocking Peptide (BLP-ZT010).

Immunohistochemistry

Expression of ZIP10 in mouse hippocampus
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal CA3 region, showed ZIP10 immunoreactivity (green) in cells of the pyramidal layer (arrows). B. Pre-incubation of the antibody with ZIP10/SLC39A10 (extracellular) Blocking Peptide (BLP-ZT010), suppressed staining. Cell nuclei are stained with DAPI (blue). P = pyramidal layer, CA3-SR = CA3 stratum radiatum

Indirect flow cytometry

Cell surface detection of ZIP10 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-APC.
^___ Cells + Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010), (2.5μg) + goat-anti-rabbit-APC.
Cell surface detection of ZIP10 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-FITC.
^___ Cells + Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010), (5μg) + goat-anti-rabbit-FITC.
Cell surface detection of ZIP10 by indirect flow cytometry in live intact human MDA-MB-231 breast adenocarcinoma cell line:
^___ Cells.
^___ Cells + goat-anti-rabbit-APC.
^___ Cells + Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010), (2.5μg) + goat-anti-rabbit-APC.

References

Kambe, T. et al. (2015) Physiol. Rev. 95, 749.
Bafaro, E. et al. (2017) Signal Transduc. Target Ther. 2, 17029.
Chen, B. et al. (2024) Signal Transduc. Target Ther. 9, 6.
Hojyo, S. et al. (2014) Proc. Nat. Acad. Sci USA. 111, 11786.
Gao, H. et al. (2017) Proc. Nat. Acad. Sci USA. 114, 12940.
Taylor, K.M. et al. (2016) Biochem. J. 473, 2531.
Nimmanon, T. et al. (2021) Cell. Mol. Life Sci. 78, 1781.
Kagara, N. et al. (2007) Cancer Sci. 98, 692.

Scientific background

ZIP10, also known as Solute Carrier Family 39 Member 10 (SLC39A10), is a zinc transporter protein that belongs to the ZIP (Zrt-, Irt-like Protein) family.^1-3

The ZIP family includes 14 members (ZIP1 to ZIP14), that play a critical role in the transport of metal ions, primarily zinc, iron, and manganese, across cell membranes. ZIP family members are primarily involved in the uptake of zinc and iron into the cytoplasm from the extracellular space or from intracellular organelles. Their function mostly increases the cytoplasmic concentration of these metals by transporting them across cellular membranes.^1-3

ZIP10 is expressed at the cell membrane and serves as a zinc importer into cells. ZIP10 expression is also modulated by the available zinc concentration: upregulated in the presence of low zinc concentrations and downregulated when zinc is present in excess. ^2,3

ZIP10 plays a central role in immune function regulation in both the adaptive and innate immune system. ZIP10 regulates B-cell and T-cell development and function as well as having a role in the modulation of the inflammatory response in macrophages. ^4,5

ZIP10 is expressed in several organs like kidney and the central nervous system, but its exact physiological function is still not clear.

Dysregulated zinc homeostasis is linked to the development and progression of several cancers and ZIP10, in particular, has garnered attention for its involvement in cancer biology in particular through its heteromer formation with ZIP6, another ZIP family member.^2,6-8

ZIP10 has been shown to be involved in the regulation of cell proliferation, cell migration and invasion and the regulation of apoptosis, in several cancer types including pancreatic, breast and prostate, suggesting that the inhibition of ZIP10 function could represent a therapeutic target in these and other tumors.^6-8

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-ZIP10/SLC39A10 (extracellular) Antibody (#AZT-010) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize ZIP10 from mouse, rat and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

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Anti-ZIP10/SLC39A10 (extracellular) Antibody

Overview

Application key:

Species reactivity key:

Applications

Citations

Specifications

Scientific Background

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