Overview
- Peptide (C)KDQEEGVTEKLQNGD, corresponding to amino acid residues 264 - 278 of mouse SLC39A14 (Accession Q75N73). Intracellular, 2nd loop.
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1-2. Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:200).
3-4. Anti-ZIP14 (SLC39A14) Antibody, preincubated with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024).
Western blot analysis of mouse heart membranes (lanes 1 and 3) and rat heart membranes (lanes 2 and 4):1-2. Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:200).
3-4. Anti-ZIP14 (SLC39A14) Antibody, preincubated with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024).
Western blot analysis of human LNCaP prostate carcinoma cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4):1-2. Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:500).
3-4. Anti-ZIP14 (SLC39A14) Antibody, preincubated with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024).
Expression of ZIP14 in mouse striatum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. ZIP14 immunoreactivity (green) appears in neuron profiles (arrows). B. Pre-incubation of the antibody with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of ZIP14 in mouse cerebellum.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ZIP14 (SLC39A14) Antibody (#AZT-024), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. ZIP14 immunoreactivity (green) appears in neuron profiles (arrows) and diffusely in the molecular layer (MOL). B. Pre-incubation of the antibody with ZIP14 (SLC39A14) Blocking Peptide (BLP-ZT024), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Nelson. N, et al (1999), EMBO Journal. 18, 4371.
- Eide.D, et al (2004), Pflugers Arch. 10, 1074.
- Aydemir.T, et al (2018), Journal of Nutrition. 174–184.
- Winslow. J, et al (2020), Department of Nutritional Sciences. 21, 3304.
Metal ions are essential elements that participate in many different biological processes and therefore their homeostasis is crucial for life. These ions are utilized in oxygen transfer, catalysis, macromolecule stability, gene expression and cell signaling. The homeostasis of metal ions in cells dictated using a specific set of transporters responsible for maintaining their appropriate concentration1.
SLC39A14 (ZIP14) is a member of the the Zrt – and – Irt like family (ZIP) a large family of metal ion transporters that can be found in organisms across all kingdoms of life. ZIP14 function as a transporter of zinc, iron, manganese and cadmium across cellular membranes2.
According to ZIP14 predicted structure it consists of 8 transmembrane domains (TMDs), extracellular N-terminus and C-terminus and several histidine reach repeats. Structural studies of ZIP14 using SDS-PAGE analysis under nonreducing conditions was show that ZIP14 undergoes trimerization3.
Human ZIP14 mRNA was detected in the liver, pancreases, thyroid and in the heart. In addition, mouse mRNA was also detected in the kidney, white adipose tissue (WAT), skeletal muscle and spleen. Mutations in gene encoding ZIP14 leads to manganese toxicity and early-onset dystonia in humans, highlighting ZIP14 important role in manganese homeostasis4.
Anti-ZIP14 (SLC39A14) Antibody (#AZT-024) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize ZIP14 from rat, mouse and human samples.
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