Name: Anti-ZIP6/SLC39A6 (extracellular)-FITC Antibody
Brand: Alomone Labs
SKU: AZT-006-F

Overview

Cat #: AZT-006-F

Alternative Name Zinc transporter ZIP6, Zrt- and Irt-like protein 6, Estrogen-regulated protein LIV-1, LIV1, Solute carrier family 39 member 6

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)ESVSEPRKSFMYSR, corresponding to amino acid residues 251-264 of mouse ZIP6 (Accession Q8C145). Extracellular, N-terminus.

Accession (Uniprot) Number Q8C145

Gene ID 106957

Homology Rat, human – 13/14 amino acid residues identical.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG

Label Fluorescein isothiocyanate (FITC)

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #AZT-006), and direct flow cytometry (labeled antibody).

Applications: fc, lci

Direct flow cytometry

Cell surface detection of ZIP6 by direct flow cytometry in live intact mouse TK-1 T-cell lymphoma cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-ZIP6/SLC39A6 (extracellular)-FITC Antibody (#AZT-006-F), (2.5µg).
Cell surface detection of ZIP6 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells:
^___ Cells.
^___ Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F).
^___ Cells + Anti-ZIP6/SLC39A6 (extracellular)-FITC Antibody (#AZT-006-F), (5µg).

References

Matsui, C. et al. (2017) FEBS Lett. 591, 3348.
Takatani-Nakase, T. (2018) Biol. Pharm. Bull. 41, 1517.
Bin, B.H. et al. (2018) J. Immunol. Res. 2018, 9365747.
Bagheri, S. et al. (2019) Monoclon. Antib. Immunodiagn. Immunother. 38, 70.
Colomar-Carando, N. et al. (2019) J. Immunol. 202, 441.
Taylor, K.M. et al. (2016) Biochem. J. 473, 2531.
Kong, B.Y. et al. (2014) Mol. Hum. Reprod. 20, 1077.

Scientific background

ZIP6 (SLC39A6) is a zinc (Zn) transporter located on the cell membrane.

Zn is an essential heavy metal, part of numerous biological processes as an enzymatic co-factor, and also as an intracellular signaling mediator that recruits extracellular stimuli and determines the final outputs of various physiological pathways. Intracellular and extracellular Zn levels regulate many cellular processes such as autophagy, cell growth and death, and cell migration^1,2.

Movement of Zn in and out of cells, across membranes, is regulated by zinc transporters. Zn transporter protein 6 (ZIP6) is a member of the ZIP family of Zinc transporters and of the LIV-1 sub-family. ZIP transporters contain 8 transmembrane domains with a cytoplasmic region between TM3 and TM4. LIV-1 subfamily members contain the HEXXH motif within TM5. They also exhibit diverse lengths of the N-terminal extracellular domain³.

The gene encoding this transporter has been shown to take part in estrogen receptor‐positive breast cancer and serves as a reliable marker of this disease. Overexpression of ZIP6 protein is also observed in prostate and kidney tumor cells⁴.

ZIP6 is also strongly upregulated during T-cell activation⁵. ZIP6 heteromerizes with ZIP10 for oocyte-to-egg transition and epithelial to mesenchymal transition in fetus development^6,7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-ZIP6/SLC39A6 (extracellular) Antibody (#AZT-006) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize ZIP6 from rat, mouse and human samples.

Anti-ZIP6/SLC39A6 (extracellular)-FITC Antibody (#AZT-006-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize ZIP6 from rat, mouse and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

Applications

Citations

Specifications

Scientific Background

Specific Control Product

Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. Isotype controls are used as negative controls to help differentiate non-specific background signal from specific antibody signal.

Our Rabbit IgG Isotype Control-FITC (#RIC-001-F), was specifically developed to be used as a negative control in surface staining flow cytometry along our line of rabbit primary surface staining antibodies conjugated to FITC.

Rabbit IgG Isotype Control-FITC (#RIC-001-F)

Shipping and Ordering Information

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Products are lyophilized and ship at room temperature - FREE OF CHARGE.

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For more country-specific shipping and contact information see Ordering & Shipping.

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Anti-ZIP6/SLC39A6 (extracellular)-FITC Antibody