Overview
Type: Synthetic peptide
Form: Lyophilized powder
Apelin/APJ Receptor (extracellular) Blocking Peptide (#BLP-AR030) is the original antigen used for immunization during Anti-Apelin/APJ Receptor (extracellular) Antibody (#AAR-030) generation. The blocking peptide binds and ‘blocks’ Anti-Apelin/APJ Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membrane (lanes 1 and 4), rat pituitary cell line (GH3) lysate (lanes 2 and 5) and rat heart membrane (lanes 3 and 6):1-3. Anti-Apelin/APJ Receptor (extracellular) Antibody (#AAR-030), (1:200).
4-6. Anti-Apelin/APJ Receptor (extracellular) Antibody, preincubated with Apelin/APJ Receptor (extracellular) Blocking Peptide (#BLP-AR030).
Expression of Apelin receptor in mouse brainImmunohistochemical staining of mouse cerebellum free floating frozen sections using Anti-Apelin/APJ Receptor (extracellular) Antibody (#AAR-030), (1:600). A. Apelin receptor staining (green) appears in neurons of the Purkinje cell (PC) (horizontal arrows in C) layer and in the dendritic tree (vertical arrow in C) in the molecular layer (MOL). B. Nuclei staining using DAPI as the counterstain (blue). C. Merged image of panels A and B.
Properties
Sequence
- DGYNYYGADNQSEC, corresponding to amino acid residues 4-17 of rat apelin receptor (Accession Q9JHG3).
Accession (Uniprot) Number Q9JHG3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
