Cat #: BLP-AR030
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- DGYNYYGADNQSEC, corresponding to amino acid residues 4-17 of rat apelin receptor (Accession Q9JHG3).
Accession (Uniprot) Number Q9JHG3
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse brain membrane (lanes 1 and 4), rat pituitary cell line (GH3) lysate (lanes 2 and 5) and rat heart membrane (lanes 3 and 6):1-3. Anti-Apelin/APJ Receptor (extracellular) Antibody (#AAR-030), (1:200).
4-6. Anti-Apelin/APJ Receptor (extracellular) Antibody, preincubated with Apelin/APJ Receptor (extracellular) Blocking Peptide (#BLP-AR030). - Mouse brain sections (1:600).
Last update: 02/01/2024