Overview
Type: Synthetic peptide
Form: Lyophilized powder
Aquaporin 2 Blocking Peptide (#BLP-QP002) is the original antigen used for immunization during Anti-Aquaporin 2 Antibody (#AQP-002) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney membranes:1. Anti-Aquaporin 2 Antibody (#AQP-002), (1:200).
2. Anti-Aquaporin 2 Antibody, preincubated with a blocking peptide.
Expression of AQP2 in rat kidneyImmunohistochemical staining of rat kidney paraffin embedded section showing the inner medulla using Anti-Aquaporin 2 Antibody (#AQP-002), (1:100). Intense stain (brown color) is present in collecting ducts but not in thin segments of the loop of Henle. Hematoxilin is used as the counterstain.- Mouse paraffin-embedded kidney sections (1:200) (Kim, J.I. et al. (2013) Am. J. Physiol. 304, F1283.).
Properties
Sequence
- (C)RQSVELHSPQSLPRGSKA, corresponding to amino acid residues 254-271 of rat AQP2 (Accession P34080).
Accession (Uniprot) Number P34080
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
