Overview
Type: Synthetic peptide
Form: Lyophilized powder
Aquaporin 3 Blocking Peptide (#BLP-QP003) is the original antigen used for immunization during Anti-Aquaporin 3 Antibody (#AQP-003) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 3 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney membranes:1. Anti-Aquaporin 3 Antibody (#AQP-003), (1:200).
2. Anti-Aquaporin 3 Antibody, preincubated with Aquaporin 3 Blocking Peptide (#BLP-QP003).
Expression of Aquaporin 3 in rat colonImmunohistochemical staining of paraffin embedded longitudinal section of rat colon using Anti-Aquaporin 3 Antibody (#AQP-003), (brown). AQP3 is detected in absorptive cells that are present in the luminal epithelium and in the superior third of the intestinal glands (arrows). Hematoxilin is used as the counterstain. Mucosa (M), submucosa (SM) and muscularis externa (ME).
Properties
Sequence
- (C)STEAENVKLAHMKHKEQI, corresponding to amino acid residues 275-292 of rat AQP3 (Accession P47862).
Accession (Uniprot) Number P47862
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
