Overview
Type: Synthetic peptide
Form: Lyophilized powder
Aquaporin 4/AQP4 (300-314) Blocking Peptide (#BLP-QP014) is the original antigen used for immunization during Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 4/AQP4 (300-314) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:1,2. Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014), (1:200).
3,4. Anti-Aquaporin 4 (AQP4) (300-314) Antibody, preincubated with Aquaporin 4/AQP4 (300-314) Blocking Peptide (#BLP-QP014).
Expression of Aquaporin 4 in rat brainImmunohistochemical staining of rat brain using Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014), (1:200). A. Aquaporin 4 (green) is detected in blood vessels in layers 1-3 of rat neocortex. B. Astrocytic processes were stained using mouse anti glial fibrillary acidic protein (GFAP) (red) and DAPI (blue) was used to delineate the cortical layers. C. Merge of Aquaporin 4 and GFAP shows overlap along blood vessels (arrows) in agreement with the role of astrocytes and Aquaporin 4 in regulation of the blood-brain barrier.
Properties
Sequence
- (C)HVIDIDRGDEKKGKD, corresponding to amino acid residues 300-314 of rat AQP4 (Accession P47863).
Accession (Uniprot) Number P47863
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
