Overview
Type: Synthetic peptide
Form: Lyophilized powder
Aquaporin 5 Blocking Peptide (#BLP-QP005) is the original antigen used for immunization during Anti-Aquaporin 5 Antibody (#AQP-005) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse salivary gland (1 and 3) and rat lung (2 and 4):1,2. Anti-Aquaporin 5 Antibody (#AQP-005), (1:200).
3,4. Anti-Aquaporin 5 Antibody, preincubated with Aquaporin 5 Blocking Peptide (#BLP-QP005).
Expression of AQP5 in rat lungImmunohistochemical staining of rat lung sections using Anti-Aquaporin 5 Antibody (#AQP-005), (left panel). The strong staining in the alveoli (red brown) is consistent with type 1 pneumocytes (arrows). Counterstain of cell nuclei appears blue. A negative control is shown in the right panel.
Properties
Sequence
- (C)DHREERKKTIELTAH, corresponding to amino acid residues 251-265 of rat AQP5 (Accession P47864).
Accession (Uniprot) Number P47864
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
