Overview
Type: Synthetic peptide
Form: Lyophilized powder
Aquaporin 6 Blocking Peptide (#BLP-QP006) is the original antigen used for immunization during Anti-Aquaporin 6 Antibody (#AQP-006) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 6 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat kidney membranes:1. Anti-Aquaporin 6 Antibody (#AQP-006), (1:200).
2. Anti-Aquaporin 6 Antibody, preincubated with Aquaporin 6 Blocking Peptide (#BLP-QP006).- Rat and mouse kidney sections.
Properties
Sequence
- (C)EPQKKESQTNSEDTEVSV corresponding to amino acid residues 259-276 of rat AQP6 (Accession Q9WTY0).
Accession (Uniprot) Number Q9WTY0
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
