Overview
Type: Synthetic peptide
Form: Lyophilized powder
Aquaporin 9 Blocking Peptide (#BLP-QP009) is the original antigen used for immunization during Anti-Aquaporin 9 Antibody (#AQP-009) generation. The blocking peptide binds and ‘blocks’ Anti-Aquaporin 9 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 4), testis (lanes 2 and 5) and liver (lanes 3 and 6) membranes:1,2,3. Anti-Aquaporin 9 Antibody (#AQP-009), (1:200).
4,5,6. Anti-Aquaporin 9 Antibody, preincubated with Aquaporin 9 Blocking Peptide (#BLP-QP009).
Western blot analysis of rat H4-II-E-C3 hepatoma cell line lysate:1. Anti-Aquaporin 9 Antibody (#AQP-009), (1:200).
2. Anti-Aquaporin 9 Antibody, preincubated with Aquaporin 9 Blocking Peptide (#BLP-QP009).
Western blot analysis of mouse brain membranes:1. Anti-Aquaporin 9 Antibody (#AQP-009), (1:200).
2. Anti-Aquaporin 9 Antibody, preincubated with Aquaporin 9 Blocking Peptide (#BLP-QP009).
Expression of AQP9 in rat liverImmunohistochemical staining of rat liver paraffin embedded sections using Anti-Aquaporin 9 Antibody (#AQP-009) (1:100). AQP9 (brown) is expressed in hepatocytes of the liver parenchyma. Hematoxilin is used as the counterstain.
Expression of AQP9 in rat epididymisImmunohistochemical staining of rat testis paraffin embedded section using Anti-Aquaporin 9 Antibody (#AQP-009), (1:100). AQP9 (brown) is expressed in the columnar epithelium of the epididymus (arrows). Hematoxilin is used as the counterstain.
Properties
Sequence
- (C)EKDGAKKSLMQRLALK, corresponding to amino acid residues 4-19 of rat AQP9 (Accession P56627).
Accession (Uniprot) Number P56627
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
