Cat #: BLP-QP009
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Properties
Sequence
- (C)EKDGAKKSLMQRLALK, corresponding to amino acid residues 4-19 of rat AQP9 (Accession P56627).
Accession (Uniprot) Number P56627
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain (lanes 1 and 4), testis (lanes 2 and 5) and liver (lanes 3 and 6) membranes:1,2,3. Anti-Aquaporin 9 Antibody (#AQP-009), (1:200).
4,5,6. Anti-Aquaporin 9 Antibody, preincubated with Aquaporin 9 Blocking Peptide (#BLP-QP009). - Western blot analysis of rat H4-II-E-C3 hepatoma cell line lysate:1. Anti-Aquaporin 9 Antibody (#AQP-009), (1:200).
2. Anti-Aquaporin 9 Antibody, preincubated with Aquaporin 9 Blocking Peptide (#BLP-QP009). - Western blot analysis of mouse brain membranes:1. Anti-Aquaporin 9 Antibody (#AQP-009), (1:200).
2. Anti-Aquaporin 9 Antibody, preincubated with Aquaporin 9 Blocking Peptide (#BLP-QP009). - Rat liver and testis sections (paraffin embedded), (1:100).
Last update: 02/01/2024