Certificate of Analysis

Ascomycin

Name: Ascomycin
Brand: Alomone Labs
SKU: A-800

Immunomycin, FK520

Immunosuppressant

Back to product page SDS

Overview

Cat #: A-800

Alternative Name Immunomycin, FK520

Lyophilized Powder yes

Origin Streptomyces hygroscopicus var. subspascomyceticus.

Source Natural

MW: 792

Purity: >99%

Effective concentration 10-100 nM.

Structure

Chemical name (1R,9S,12S,13R,14S,17R,18E,21S,23S,24R,25S,27R)- 17-ethyl-1,14-dihydroxy-12-[(1E)-1-[(1S,3S,4S)-4-hydroxy-3-methoxycyclohexyl]prop-1-en-2-yl]-23,25-dimethoxy-13,19,21,27-tetramethyl-11,28-dioxa-4-azatricyclo[22.3.1.0^{4,9}]octacos-18-ene- 2,3,10,16-tetrone.

Molecular formula C₄₃H₆₉NO₁₂.

CAS No.: 11011-38-4

Activity Ascomycin is an immunosuppressant antifungal antibiotic^1,2. It also inhibits calcineurin (PP-2B) activity³.

References-Activity

Arai, T. et al. (1962) J. Antibiot. (Tokyo) 15, 231.
Morisaki, M. and Arai, T. (1992) J. Antibiot. (Tokyo) 45, 126.
Meadows, R.P. et al. (1993) Biochemistry 32, 754.

Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Solubility DMSO, methanol or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions Up to four weeks at 4°C or three months at -20°C.

Our bioassay

Alomone Labs Ascomycin inhibits the proliferation of Jurkat cells.
Cells were treated with different concentrations of Ascomycin (#A-800) for 4 days. The number of living cells was measured by XTT and normalized to the control (100%) and plotted against the drug concentration.

Scientific background

Ascomycin (FK520) is a structurally complex macrolide with immunosuppressant activity. It is an ethyl analog of the known immune suppressant FK506 (Tacrolimus).^1,2 Ascomycin acts by binding to a class of cytosolic proteins, the immunophilins, especially macrophilin-12 (FKBP-12). The immunophilin-FK520 complex inhibits calcineurin, a type 2B phosphatase (PP2B).³

Ascomycin suppresses the production of Th1 and Th2 cytokines in T lymphocytes, and preferentially inhibits the activation of mast cells (principal cells of the allergic response).⁴ In human basophils, it potently inhibited anti-IgE-induced histamine and cytokine release, and reduced IgE-dependent p38 MAPK activation.⁵ It seems that Ascomycin inhibits basophil degranulation at the initial phase of allergic skin reactions.^6,7 Ascomycin derivatives are currently developed for the clinical treatment of dermatological diseases.^8,9

Lyophilized Powder

For research purposes only, not for human use

Last Update: 07/05/2024

Specifications

Citations

Applications

Scientific Background

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.

Reagent Rewards

Not sure whether the product fits your experimental setup?

Try it first!

Alomone Guarantee

We offer 100% guarantee on all our products. If our products do not perform as stated, please contact our technical support staff.

Alomone Guarantee information

Navigation

Order Summary