Overview
Type: Synthetic peptide
Form: Lyophilized powder
ASCT1/SLC1A4 Blocking Peptide (#BLP-NT081) is the original antigen used for immunization during Anti-ASCT1 (SLC1A4) Antibody (#ANT-081) generation. The blocking peptide binds and ‘blocks’ Anti-ASCT1/SLC1A4 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-ASCT1 (SLC1A4) Antibody (#ANT-081), (1:200).
3,4. Anti-ASCT1 (SLC1A4) Antibody, preincubated with ASCT1/SLC1A4 Blocking Peptide (#BLP-NT081).
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human Colo 205 colon adenocarcinoma cell line lysate (lanes 2 and 4):1,2. Anti-ASCT1 (SLC1A4) Antibody (#ANT-081), (1:200).
3,4. Anti-ASCT1 (SLC1A4) Antibody, preincubated with ASCT1/SLC1A4 Blocking Peptide (#BLP-NT081).
Expression of ASCT1 in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ASCT1 (SLC1A4) Antibody (#ANT-081), (1:300), followed by goat-anti-rabbit-AlexaFluor-488. ASCT1 staining (green) is detected in the sub-granular layer (SGL, arrows) of the dentate gyrus. GL = granule layer. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)SKSEEETSPLVTH, corresponding to amino acid residues 500-512 of mouse SLC1A4 (Accession O35874).
Accession (Uniprot) Number O35874
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
