Overview
Cat #: BLP-NT082
Type: Synthetic peptide
Form: Lyophilized powder
ASCT2/SLC1A5 Blocking Peptide (#BLP-NT082) is the original antigen used for immunization during Anti-ASCT2 (SLC1A5) Antibody (#ANT-082) generation. The blocking peptide binds and ‘blocks’ Anti-ASCT2/SLC1A5 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB- Western blot, IHC- Immunohistochemistry
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):1,2. Anti-ASCT2 (SLC1A5) Antibody (#ANT-082), (1:200).
3,4. Anti-ASCT2 (SLC1A5) Antibody, preincubated with ASCT2/SLC1A5 Blocking Peptide (#BLP-NT082). - Expression of ASCT2 in mouse hippocampusImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASCT2 (SLC1A5) Antibody (#ANT-082), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ASCT2 staining appears in a layer (arrows) adjacent to the dentate granule layer (GL). Cell nuclei are stained with DAPI (blue).
Properties
Sequence
Accession (Uniprot) Number P51912
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.