Overview
Alomone Labs is pleased to offer the ASIC Channel Antibody Explorer Kit (#AK-226). This Explorer Kit includes antibodies targeting ASIC channels along with their respective peptide control antigen. An ideal tool for screening purposes.
Compounds
| Product Name | Cat # | Size |
|---|---|---|
Anti-ASIC1 Antibody |
ASC-014 | 1 x 50 µl |
ASIC1 Blocking Peptide |
BLP-SC014 | 1 x 40 µg |
Guinea pig Anti-ASIC1 Antibody |
ASC-014-GP | 1 x 50 µl |
ASIC1 Blocking Peptide |
BLP-SC014 | 1 x 40 µg |
Anti-ASIC2a Antibody |
ASC-012 | 1 x 50 µl |
ASIC2a Blocking Peptide |
BLP-SC012 | 1 x 40 µg |
Anti-ASIC3 Antibody |
ASC-018 | 1 x 50 µl |
ASIC3 Blocking Peptide |
BLP-SC018 | 1 x 40 µg |
Guinea pig Anti-ASIC3 Antibody |
ASC-018-GP | 1 x 50 µl |
ASIC3 Blocking Peptide |
BLP-SC018 | 1 x 40 µg |
Anti-ASIC4 Antibody |
ASC-015 | 1 x 50 µl |
ASIC4 Blocking Peptide |
BLP-SC015 | 1 x 40 µg |
Anti-ASIC5 (extracellular) Antibody |
ASC-032 | 1 x 50 µl |
ASIC5 (extracellular) Blocking Peptide |
BLP-SC032 | 1 x 40 µg |
Anti-pan ASIC (extracellular) Antibody |
ASC-031 | 1 x 50 µl |
Pan ASIC (extracellular) Blocking Peptide |
BLP-SC031 | 1 x 40 µg |
Scientific Background
Acid-sensing ion channels (ASICs) are Na+ channels activated by external protons. ASIC4 is a member of the ASIC family that includes another three members: ASIC1, ASIC2 and ASIC3. The ASIC family is in fact part of a large channel superfamily known as degenerin/epithelial Na+ channels (DEG/ENaC) and share the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini.
The functional ASIC channel is composed of 4 subunits, which can form either a homo or heterotetramer. The subunit composition of the tetrameric channel will determine its biophysical properties such as pH sensitivity, inactivation kinetics and channel pharmacology. In fact, the ASIC4 protein does not appear to function as a proton-gated channel when expressed alone in heterologous systems and therefore a modulatory role for this subunit has been proposed.
- Grunder, S. et al. (2000) Neuroreport 11, 1607.
- Akopian, A.N. et al. (2000) Neuroreport 11, 2217.
- Kellenberger, S. et al. (2002) Physiol. Rev. 82, 735.
