Cat #: ESP-200_PACK
Size: 8 Vials
Form: Lyophilized
Antibodies
Anti-ASIC1 Antibody
Cat #: ASC-014
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IH, IP, WB
Reactivity:
H, M, R
Immunogen
Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession P55926). Intracellular, C-terminus.
Homology
Human - identical; mouse - 19/20 amino acid residues identical.
Guinea pig Anti-ASIC1 Antibody
Cat #: ASC-014-GP
Size:
0.2 ml
Host: Guinea pig
Type: Polyclonal
Application:
IC, IF, IH, WB
Reactivity:
H, M, R
Immunogen
Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession P55926). Intracellular, C-terminus.
Homology
Human - identical; mouse - 19/20 amino acid residues identical.
Anti-pan ASIC (extracellular) Antibody
Cat #: ASC-031
Size:
0.2 ml
Host: Rabbit
Type: Polyclonal
Application:
IC, IF, IFC, IH, LCI, WB
Reactivity:
H, M, R
Immunogen
Peptide (C)TRYGKELSMVKIPSK, corresponding to amino acid residues 368-382 of rat ASIC1 (Accession P55926), amino acid residues 367–381 of rat ASIC2 (Accession Q62962), amino acid residues 375–388 of rat ASIC3 (Accession O35240) and amino acid residues 378–390 of rat ASIC4 (Accession Q9JHS6). Extracellular loop.
Homology
ASIC1: Mouse, rat, human – identical.
ASIC2: Mouse, rat, human – 14/15 amino acid residues identical.
ASIC3: Mouse, rat, human – 12/15 amino acid residues identical.
ASIC4: Mouse, rat – 12/15 amino acid residues identical; human – 11/15 amino acid residues identical.
Pharmacological Reagents
MitTx Toxin
Cat #: M-100
Size:
10 µg
MW: 20.8 kDa (α subunit, 7.1 kDa and β subunit, 13.7 kDa)
Purity: >98% (HPLC)
Net peptide content: 100%
Origin
Natural protein isolated from Micrurus tener tener (Texas coral snake).
Effective concentration
20 nM
Activity
MitTx toxin activates ASIC1-containing channels1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Water or any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.
Psalmotoxin-1
Cat #: STP-200
Size:
0.1 mg
MW: 4689.5 Da
Purity: >99% (HPLC)
Net peptide content: 100%
Effective concentration
1-10 nM.
Activity
Psalmotoxin-1 inhibits cation currents mediated by acid-sensing ion channels (ASIC1a)1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.
Antibody Storage & Reconstitution Instructions
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Add deionized water, depending on the sample size.
Storage after reconstitution
The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control antigen storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution
100 μl water.
Control antigen storage after reconstitution
-20ºC.
Preadsorption control
1 μg peptide per 1 μg antibody.
For more information, please refer to individual certificate of analysis for each product.
Note
A guinea pig polyclonal antibody (#ASC-014-GP) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
